COMBINED PRETREATMENT OF HOT COMPRESSED WATER AND WET DISK MILLING UNRAVELED OIL PALM BIOMASS STRUCTURES AND ENHANVED ENZYMATIC DIGESTIBILITY

摘要

In the present study, hot compressed water (HCW) pretreatment, wet disk milling (WDM) pretreatmentrnand its combination were performed with the aim to reduce natural recalcitrant of lignocellulosic biomass and providernmaximal access to cellulase attack. Oil palm frond fiber (OPFF) and oil palm empty fruit bunch (OPEFB) werernhydrothermally pretreated at reaction temperature and time ranges from 150℃ to 190℃ and 10 to 20 min,rnrespectively. Subsequent treatment with WDM resulted in fibrillation of fiber into nanofibers size thus increasing thernconversion of cellulose to glucose. The effects of combined HCW and WDM pretreatments were evaluated byrnchemical composition changes, power consumption, particle size and morphological alterations by SEM. Thernsuccessful of pretreatments were assessed by the degree of enzymatic digestibility of treated samples. Approximatelyrnabout 66.2% and 55.8% of xylose yield were obtained from OPFF and OPEFB samples, respectively. The highestrnglucose yield obtained from OPFF and OPEFB samples under combined pretreatment were 80.5% and 90.3%,rnrespectively which only consumed about 17.8-19.1 MJ/kg of biomass.