Thermosensitive magnetic particles as solid phase support in an immunoassay

摘要

The adsorption and the chemical grafting of an antibody (anti-Alpha-foeto protein) onto thermosensitive magnetic latex particles has been investigated as a function of pH, ionic strength and temperature. The hydrophilic magnetic latexes bearing surface carboxylated groups have been prepared on the basis of an heterocoagulation concept. The adsorption of the antibody (anti-Alpha-foeto protein) was found to drastically decreased upon increasing the pH, whereas, the effect of ionic strength was found to be negligible in the studied range. The covalent coupling of the antibody was performed using two cardodiimide and succinimide derivatives. The prepared conjugates (latex-antibody) were evaluated by performing the classical Enzyme Linked Immune Sorbent Assay (ELISA) for detecting the Alpha-foeto protein (AFP). [References: 13]