摘要:
为探讨有氧运动强度对斜带石斑鱼(Epinephelus coioides)生长、血液非特异性免疫机能和肝脏抗氧化能力的影响,本研究将斜带石斑鱼放置在4个运动强度(对照组0 bl/s、实验组0.5 bl/s、实验组1.0 bl/s和实验组2.0 bl/s)中进行8周的训练实验.结果表明,1.0 bl/s运动强度对斜带石斑鱼的特定生长率(SGR)、增重率(WGR)和成活率(SR)有显著提高作用(P<0.05).血清中总蛋白(TP)、球蛋白(GLB)、补体C3含量和溶菌酶(LYZ)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)活性随运动强度的增加先上升后下降,其中1.0 bl/s组显著高于其他组(P<0.05),而血清谷丙转氨酶(GPT)和谷草转氨酶(GOT)活性先下降后上升,其中1.0 bl/s组显著低于其他组(P<0.05),白蛋白(ALB)和补体C4含量无显著性差异(P>0.05).肝脏总抗氧化能力(T-AOC)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)的活性随运动强度的增加也呈现先增加后降低的趋势,在1.0 bl/s组中活性显著高于其他组(P<0.05).8周运动训练对肝脏HSP70mRNA表达水平有显著影响,1.0 bl/s组显著高于其他组(P<0.05).总之,1.0 bl/s运动强度可提高斜带石斑鱼幼鱼的生长速度、增强血液非特异性免疫功能和肝脏抗氧化能力,上调HSP 70 mRNA表达水平.%The present study was conducted to investigate the effects of exercise training on the growth,serum innate immunity,and hepatic antioxidant capacity of orange-spotted grouper (Epinephelus coioides).The fish were exercised at an intensity of 0,0.5,1,and 2 body lengths per second (bl/s) for 8 weeks.Specific growth,weight gain,and survival increased significantly in the 1 bl/s group (P<0.05).The plasma concentrations of total protein,globulin protein,complement 3,lysozyme,alkaline-phosphatase,and acid phosphatase increased as exercise intensity increased up to 1 bl/s and then decreased at the higher intensity of 2 bl/s (P<0.05).The plasma contents of glutamic-pyruvic transaminase and glutamic-oxaloacetic transaminase decreased as the exercise intensity increased up to 1 bl/s,and then increased at the higher intensity of 2 bl/s (P<0.05).The albumin and complement 4 contents of plasma did not significantly differ among treatments.Hepatic total antioxidant capacity,catalase,and superoxide dismutase activity increased with exercise intensity up to 1 bl/s and then decreased at 2 bl/s (P<0.05).The exercise treatments significantly affected the mRNA expression of HSPs70,and that of the 1 bl/s group was the highest.The results indicated that an exercise intensity of 1 bl/s effectively enhanced the growth,serum innate immunity,hepatic antioxidant capacity,and HSPs70 mRNA expression of Epinephelus coioides.