摘要：
Rapid and accurate detection of seed vigor,was the premise of agricultural production.However,the traditional determination methods operated troublesomely,and caused irreversible damage to the seed vigor.In order to develop a rapid,nondestructive seed vigor determination for Brassica plants,the artificially aged seeds of Brassica pekinensis,B.juncea,B.parachinensis seeds were used as experimental materials,and the standard germination test was performed to obtain its seed vigor.In addition,the accuracy,convenience and injury for tested seed of electrical conductivity method,optical density method and near infrared spectroscopy,in seed vigor determination were compared.The results showed the three methods could detect seeds vigor considerable accurately.On the other hand electrical conductivity method and optical density method could determine seed vigor more rapid than standard germination test,but still took more than one day.Further,the measured value was easily influenced by environmental factors,damaged to tested seed,and operation cumbersome.Fortunately,the method developed by near infrared spectroscopy was demonstrated to be rapid,nondestructive,and simple to determine seed vigor determination for Brassica plants.%快速准确地检测种子活力,是农业增产的前提,而传统的测定方法操作繁琐,且对种子活力造成不可逆的损害.为建立快速、无损的芸薹属植物种子活力检测方法,以经过人工老化的白菜、芥菜、菜心等蔬菜种子为实验材料,利用标准发芽试验获得其准确的种子活力数据.然后,对比电导率法、光密度法和近红外光谱法在测定芸薹属种子活力中的准确性、快捷性以及对所测种子的损伤程度.结果表明,三种方法均能比较准确的检测芸薹属植物种子活力,虽然电导法和光密度法测定种子活力比标准发芽试验快,但依然需要1天时间,而且结果受环境影响大,种子造成损伤,操作繁琐.而使用近红外技术建立的模型可更准确地预测种子活力,相关系数在0.95以上,用时仅需要若干分钟,而且对种子没有损伤.
