coffee

摘要： The processability and ultimate quality of coffee(Coffea arabica)are determined by the composition of the matured fruits.The basis of genetic variation in coffee fruit quality could be explained by studying color formation during fruit maturation.Transcriptome profiling was conducted on matured fruits of four C.arabica varieties(orange colored fruits(ORF);purple colored fruits(PF);red colored fruits(RF)and yellow colored fruits(YF))to identify key color-regulating genes,biosynthesis pathways and transcription factors implicated in fruit color formation.A total of 39,938 genes were identified in the transcriptomes of the four C.arabica varieties.In all,2745,781 and 1224 differentially expressed genes(DEGs)were detected in YF_vs_PF,YF_vs_RF and YF_vs_ORF,respectively,with 1732 DEGs conserved among the three pairwise groups.Functional annotation of the DEGs led to the detection of 28 and 82 key genes involved in the biosynthesis of carotenoids and anthocyanins,respectively.Key transcription factors bHLH,MYB,NAC,MADS,and WRKY implicated in fruit color regulation were detected.The high expression levels of gene-LOC113688784(PSY),gene-LOC113730013(b-CHY),gene-LOC113728842(CCD7),gene-LOC113689681(NCED)and gene-LOC113729473(ABA2)in YF may have accounted for the yellow coloration.The differential expression of several anthocyanin and carotenoid-specific genes in the fruits substantially account for the purple(PF),red(RF),and orange(ORF)colorations.This study provides important insights into fruit color formation and variations in C.arabica and will help to develop coffee varieties with specific color and quality traits.

摘要： Members of the ERF Family of Transcription Factors play an important role in plant development and gene expression that regulates responses to biotic and abiotic stress.This work identified 36 ERF family genes in Coffea arabica within the AP2/ERF full domain,using the EST-based genomic resource of the Brazilian Coffee Genome Project.The ERF family genes were classified into nine of the ten existing groups through phylogenetic analysis of the deduced amino acid sequences and comparison with the sequences of the ERF family genes in Arabidopsis.In addition to the AP2 domain,other conserved domains were identified,typical of members of each group.The in silico analysis and expression profiling showed high levels of expression for libraries derived from tissues of fruits,leaves and flowers as well as for libraries subjected to water stress.These results suggest the participation of the ERF family genes of C.arabica in distinct biological functions,such as control of development,maturation,and responses to water stress.The results of this work imply in the selection of promising genes for further functional characterizations that will provide a better understanding of the complex regulatory networks related to plant development and responses to stress,opening up opportunities for coffee breeding programs.

摘要： BACKGROUND Previous systematic reviews have consistently reported that coffee consumption has a preventive effect on the occurrence of type 2 diabetes mellitus(T2DM).However,further evaluations between coffee consumption and the risk of T2DM in Asian populations are needed.AIM To conduct a meta-epidemiological study on systematic reviews evaluating the association between coffee consumption and the risk of T2DM in Asian people.METHODS The selection criterion was defined as a population-based prospective cohort study evaluating the association between coffee consumption and the risk of T2DM in Asian populations,reporting the adjusted relative risk(RR)and its 95%confidence interval(CI)for potential confounders.A fixed-effect model metaanalysis was applied to calculate the summary RR and its 95%CI in less than 50%of the I2 value indicating the level of heterogeneity.A two-stage fixed-effects doseresponse meta-analysis(DRMA)was performed to calculate the risk per unit dose(a cup per day).RESULTS A total of seven studies were selected in this meta-epidemiological study.The risk of T2DM in Asian populations was significantly reduced in the highest to the lowest dose group(summary RR=0.73,95%CI:0.66-0.82;I2 value=0.0%).The DRMA showed that drinking one cup of coffee per day reduced the risk of T2DM in Asian populations by 8%(RR=0.92,95%CI:0.90-0.95).CONCLUSION These findings support the conclusion that coffee consumption has a protective effect on the occurrence of T2DM in Asian men and women.

摘要： The present study aimed to develop a protocol for somatic embryogenesis and encapsulation of coffee embryos(Coffea arabica L.),for the conservation of genotypes with characteristics of commercial interest.Somatic embryos were induced from leaf explants in Murashige and Skoog medium(MS)supplemented with 1 mg·L^(−1) of 2,4-dichlorophenoxiacetic acid(2,4-D)combined with 2 mg·L^(−1) of benzyladenine(BA).Somatic embryos(SE)at the globular stage were encapsulated in a sodium alginate matrix;two treatments were tested:MS+5 mg·L^(−1) BA+1 mg·L^(−1) NAA+3%(w/v)alginate,and MS+7 mg·L^(−1) BA+5.7 mg·L^(−1) indoleacetic acid(IAA)+3%(w/v)alginate.Alginate was complexed with 100 mM calcium chloride(CaCl_(2)).Viability of the encapsulated SE was determined by staining with 0.01%fluorescein diacetate(FDA)after 0,15,30,and 45 days of storage at 4°C.Embryo viability was 100%in both treatments.

摘要： Worldwide the issue of mycotoxins results in economic losses estimated at billions of dollars and toxicological risk for both humans and animals.Preventive measures also include decontamination and mitigation actions that can be carried out through food processing.Several proposals have been tested and illustrated also in scientific papers during the last decades,however clear,easy to implement,practical suggestions and guidelines for process adaptation are much more needed.Europe and South East Asia can find synergies and complementarities moving from processing to analysis,from risk assessment to reduction strategies,from gap-analysis to communication roadmaps.Stakeholders from both Europe and Southeast Asia must then ensure that there is a way to ease and harmonize the regulation in the food supply chain in order to ensure food safety and at the same time facilitate trade in both regions,taking into account of the various landscapes,agrosystems and also different consumer preferences within the countries themselves.Concerning the example of cereals,processing steps cover primary processing(cleaning and milling operations)and secondary processing procedures(such as fermentation and thermal treatments during baking),special attention is devoted to the production of baked goods and to the estimation of processing factors for DON in wheat bread production especially in Europe.With reference instead to the specific context of Asian producers,a case-study focuses on ochratoxin A in coffee in Southeast Asia region,combining the expertise of the farming community,coffee industry and science researchers.The strategy to reduce mycotoxins in a farm setting poses several challenges to coffee farmers:it needs to be analyzed in the context of good agricultural practices,socioeconomic and behavioral factors of both coffee producers and consumers.As the world becomes more globalized,food and feed supply chains also become more complexed and hence,a more comprehensive strategy to ensure food contaminants mitigation is needed.

摘要： 从coffee传入中国至今的28种汉译形式的嬗变入手,探究信、达、雅,音、形、义,情、理、法的翻译原则,以求归纳出外来词汉译形式变化与社会文化发展之间的相关规律.认为本土化是一个过程,是一个事物为了适应当前所处的环境而作的变化,也就是人们常说的人乡随俗.以黄伯荣、廖旭东《现代汉语》中对外来词的分类为依据,暂且把意译词归类为非外来词,将不同历史阶段coffee的多种汉译形式分为音译、部分音泽部分意译或者音意兼泽、音译后加注汉语语素、借形4类进行统计归纳,并探求其特征.

摘要： 从coffee传入中国至今的28种汉译形式的嬗变入手,探究信、达、雅,音、形、义,情、理、法的翻译原则,以求归纳出外来词汉译形式变化与社会文化发展之间的相关规律。认为本土化是一个过程,是一个事物为了适应当前所处的环境而作的变化,也就是人们常说的入乡随俗。以黄伯荣、廖旭东《现代汉语》中对外来词的分类为依据,暂且把意译词归类为非外来词,将不同历史阶段coffee的多种汉译形式分为音译、部分音译部分意译或者音意兼译、音译后加注汉语语素、借形4类进行统计归纳,并探求其特征。

摘要： Background: Green coffee as a functional food has an antioxidant effect, which can reduce the cancer incidence, promote weight-loss and improve glucose and lipid metabolism, as well as anti-oxidant and anti-inflammatory activity. Objective: Assessing the effect of green coffee administration on experimental cisplatin induced renal apoptosis in adult male albino rats of local strain. Design: Randomized Block Design was used. Materials and Methods: Forty adult male albino rats of local strain were randomly divided into four groups of 10 animals each. G1: control negative group was received isotonic saline (0.5 ml, i.p.) for 30 consecutive days, G2: sham operated group mice were received green coffee extract in water (40 mg/kg/day) for 30 consecutive days, animals of G3 and G4 were administered cisplatin (8 mg/kg/day, i.p.) at 10th day, which is well known to produce significant nephrotoxicity in rats. Animals in G3 were received green coffee extract in water (40 mg/kg/day) and G4 was received isotonic normal saline (0.5 ml, i.p.) for 30 consecutive days. All rats were sacrificed after 30 days and blood was withdrawn for biochemical examinations of kidney function tests (blood urea nitrogen, creatinine and uric acid). Kidneys were removed for determination of renal oxidative stress markers (H2O2) biochemically and caspase-3 by Immunohistochemical examination. Results: Cisplatin administration was associated with significant higher levels of BUN, creatinine, uric acid and H2O2 as compared with normal control group. Green coffee administration in cisplatin-induced renal apoptosis groups produced significant lower levels of BUN, creatinine, uric acid and H2O2 (24.4 ± 4.14, 1.730 ± 0.2830, 5.50 ± 0.850 and 0.51 ± 0.12 respectively) as compared with cisplatin-induced renal apoptosis group not administrated green coffee (27.4 ± 6, 2.04 ± .31, 7.00 ± 1.25 and 1.1 ± 0.16 respectively). Cisplatin administration increased expression of the apoptotic protein caspase-3. In contrast, treatment with green coffee extract attenuated apoptosis. Conclusion: green coffee reduced cisplatin-induced renal apoptosis. Green coffee improved the general condition of cisplatin-induced renal apoptosis rats due to its antioxidant and anti-apoptotic effects.

摘要： Effective utilization of coffee wastes has been a great challenge in Uganda despite their wider use to replenish soil organic matter. This study explored the possibility of producing biochar from coffee husks that could be used as a soil amendment for replenishing soil nutrients and also for enhancement of soil water holding capacity. Pyrolysis of coffee husks was done in a batch bio-reactor under slow pyrolysis conditions of temperatures 350°C - 550°C and residence times 30 - 60 min. For easy characterization, biochar was grinded, sieved through a 1 mm sieve and then analyzed using a computerized Thermo Graphic Analyzer with an inbuilt and integrated ELTRA 84 GmbH Precision Digital weighing scale. Proximate analysis (wet basis) of biochar gave a moisture content of 5.2%, ash content of 14.7%, volatile matter of 13.2% and fixed carbon of 66.9%. Biochar was applied to soil at different rates (0%, 5%, 10% and 20% w/w) and its effect on water holding capacity was investigated. Results show that bio-char amended soils had higher water holding capacity (p ≤ 0.05) compared to biochar free soils. The water holding capacity also increased with increase in biochar amendment with a 1.5% increase in soil water holding capacity for each 1% increase in biochar application rate. Biochar was also rich in soil nutrient elements with 0.96% N, 0.39% P and 1.97% K;this increased the availability of soil nutrients for crop growth. The results suggest that biochar could be a better tool to improve soil conditions thus enhancing the sustainability of agriculture.