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星斑川鲽

星斑川鲽的相关文献在2006年到2021年内共计104篇,主要集中在水产、渔业、动物学、遗传学 等领域,其中期刊论文69篇、会议论文23篇、专利文献24497篇;相关期刊34种,包括海洋与湖沼、水生生物学报、河北渔业等; 相关会议10种,包括中国高端养鱼模式构建与可持续发展论坛、2014年全国海水养殖学术研讨会、2013年全国海水养殖学术研讨会等;星斑川鲽的相关文献由148位作者贡献,包括王波、王际英、张利民等。

星斑川鲽—发文量

期刊论文>

论文:69 占比:0.28%

会议论文>

论文:23 占比:0.09%

专利文献>

论文:24497 占比:99.63%

总计:24589篇

星斑川鲽—发文趋势图

星斑川鲽

-研究学者

  • 王波
  • 王际英
  • 张利民
  • 刘振华
  • 孙永智
  • 丁立云
  • 姜美洁
  • 郑风荣
  • 帅继祥
  • 孙丽慧
  • 期刊论文
  • 会议论文
  • 专利文献

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    • 孙永智; 郝甜甜; 王际英; 李宝山; 黄炳山; 刘京熙
    • 摘要: 星斑川鲽(Platichthys stellatus)是我国北方重要的海水经济养殖品种,具有广温、广盐、抗逆性强、生长快等优点,且营养价值较高。目前,国内外有关该鱼的营养需求及饲料研究已经有一些报道,但是对某些营养素的研究还是空白。本文综述了星斑川鲽对蛋白质、氨基酸、脂肪、脂肪酸、碳水化合物和微量营养素的需求,蛋白源和脂肪源替代鱼粉和鱼油,功能性饲料添加剂应用等方面的研究进展,旨在为星斑川鲽的精准营养研究及优质高效配合饲料的开发提供参考资料,从而推动其养殖业的健康可持续发展。
    • 周江; 王丽娟; 王波; 郑凤荣; 王长海
    • 摘要: To investigate the relationship of genes expression between brain tissue and ovary development, and explore more functional genes of Platichthys stellatus, total RNA from the mixed sample of pituitary gland and hypothalamus at the ovary maturation and degradation stages were isolated and sequenced by HiSeq 2000 high throughput sequen-cing technology. After assembly and splicing, 24128 unigenes from a total of 30640 unigenes were annotated based on the blast homology alignment. All 29137 unigenes with eggNog annotations were divided into 26 categories that were involved in physiological processes, such as signaling transduction, translation mechanism, etc. The results from KEGG pathway analysis showed that all unigenes at the ovary maturation stage were involved in metabolic pathways with 135 up-regulated sequences, while the unigenes at the ovary degradation stage were associated with 192 metabolic path-ways with 648 up-regulated sequences. Moreover, 334 differentially expressed genes (DEGs) were up-regulated at the ovary maturation stage, while 987 up-regulated DEGs were observed at the ovary degradation stage. Total 408 uni-genes were involved in reproductive and endocrine regulation, and 1508 unigenes were involved in signaling mo-lecules. The expression of some reproduction-associated DEGs including growth hormone releasing hormone (GnRH), neurokinin B (NKB), gonadotropin (GtH), follicle stimulating hormone (FSH), kisspeptin (Kiss), and prolactin-relea-sing peptide receptor (PrRPR) were verified by qRT-PCR. All genes except FSH were consistent with the results of the transcriptome sequencing.%为了解星斑川鲽(Starry Flounder,Platichthys stellatus)脑组织基因表达与卵巢发育调控的关系,发掘相关功能基因,研究提取卵巢成熟期和退化期星斑川鲽雌鱼脑垂体和下丘脑组织总RNA,运用HiSeq 2000高通量测序技术进行转录组测序分析.测序结果经拼接组装后共获得30640条Unigenes,Blast同源性比对显示,其中24128条Unigenes获得注释;经eggNog功能注释后29137条Unigenes序列分为26类,分别涉及信号转导、翻译机制等生理生化过程.KEGG pathway数据比对显示,卵巢成熟期涉及98种代谢途径,135条序列表达上调;卵巢退化期涉及192种代谢途径,648条序列表达上调.Unigenes表达量及表达差异分析表明在卵巢成熟期334条序列表达上调,卵巢退化期987条序列表达上调.获得功能注释的Unigene中,408条涉及生殖调控和内分泌调控,参与生殖调控的信号分子有1508条.试验采用实时定量PCR研究了涉及生殖调控和内分泌调控基因促性腺激素释放激素(GnRH)、神经激肽B(NKB)、促性腺激素(GtH)、促滤泡激素(FSH)、肿瘤转移抑制因子(Kiss)以及催乳素释放肽受体(PrRPR)在卵巢两个不同发育时期脑垂体和下丘脑的表达情况,结果表明除FSH外,其余均在卵巢退化期时期脑组织中表达升高,与转录组测序结果趋势一致.
    • 段会敏; 陈松林; 田永胜; 李文龙; 李振通; 陈张帆; 唐江; 成美玲; 郭华; 孙宗哲
    • 摘要: 通过紫外线(UV)对冷冻的鲈(Lateolabrax japonicus)精子进行灭活,利用冷休克和压力休克方法诱导星斑川鲽(Platichthys stellatus)雌核发育二倍体,同时利用灭活鲈精子制备单倍体胚胎,未灭活鲈精子受精制备杂交胚胎,星斑川鲽精子受精制备正常发育胚胎.对以上几种胚胎发育时序、发育生物学特征进行了观察比较.结果表明,卵裂期单倍体、杂交二倍体和雌核发育二倍体胚胎发育速度与普通二倍体胚胎没有明显差异,从低囊胚期开始各实验组胚胎发育速度均慢于普通二倍体胚胎;杂交胚胎在胚体形成期基本死亡,单倍体胚胎在尾芽期停止发育死亡,均不能正常孵出.雌核发育二倍体与普通二倍体具有相似的发育时序,普通二倍体100 h 10 min孵化出膜,而雌核发育二倍体104 h 50 min孵化出膜.雌核发育胚体畸形率(53.59±0.36)%,孵化率(0.11±0.01)%;普通二倍体胚体畸形率(35.11±6.19)%,孵化率(58.01±5.30)%;与普通二倍体相比,雌核发育二倍体胚体畸形率高,孵化率低,但孵化鱼苗能够正常发育,获得了雌核发育群体.本研究为星斑川鲽雌核发育提供了技术方法,同时为单倍体、杂交和雌核发育胚胎的发育生物学研究提供了细胞生物学证据.%Artificially induced gynogenesis is a major technique used for controlling the sex ratio of aquatic ani-mals as well as for culturing selectively bred colonies. In this study, cryopreserved sperm of Japanese seabass Lateolabrax japonicus was UV-irradiated and used to fertilize eggs of starry flounderPlatichthys stellatus and diploid gynogenesis was induced using cold and hydrostatic pressure shocks. Meanwhile,P. stellatuseggs fertil-ized with UV-irradiatedL. japonicas sperm but without cold or hydrostatic pressure shock were used as the hap-loid group;P. stellatus eggs fertilized withL. japonicus sperm were used for hybridization; andP. stellatus eggs fertilized withP. stellatus sperm were used as a control group. The results showed that cell division started at a similar point in time in the control group as in the gynogenetic haploids and hybridized embryos. However, as compared with the controls, embryonic development among each of the three experimental groups was retarded from the late blastula stage. All the hybridized embryos died before formation of an embryonic body; all the hap-loid embryos died before the heart started to beat. Thus, none of the embryos in these two groups could be suc-cessfully hatched. Embryonic development of a small portion of the gynogenetic diploids followed a pattern re-sembling that of the controls, although with less success: (58.01±5.30)% of embryos in the control group were hatched out at 100 h 10 min, with (35.11±6.19)% malformed; in comparison, only (0.11±0.01)% of the gynoge-netic diploids were hatched out at 104 h 50 min, with (53.59±0.36)% malformed. Although the gynogenetic dip-loids had a very low hatching rate and a relatively high rate of malformation, we were still able to construct a gy-nogenetic colony ofP. stellatus with normally developing larvae. Hence, we propose that this study uncovers a promising technique forP. stellatusgynogenesis, and the results provide abundant cytobiological evidence con-tributing to information on the developmental biology of haploid, hybridized, and gynogenetic embryos of this species.
    • 张燕; 乔洪金; 李宝山; 孙永智; 公绪鹏; 王际英; 张利民
    • 摘要: A 90-day feeding trial was conducted to investigate the effects of fish oil substitution with microalgae meals on growth performance, tissue proximate composition, and biochemical indices in juvenile starry flounder, Platichthys stellatus (initial body weight 7.35 g±0.03 g). Four groups of isonitrogenous and isolipidic diets were formulated using different primary lipid sources: fish oil (FO), Schizochytrium meal (SO), Nannochloropsis meal (NO), and the mixture of these two algae meals (MO), with the remaining part filled with corn oil. Results showed that growth performance was not significantly different between FO and SO, whereas specific growth rate (SGR), protein efficiency ratio (PER), and viscerosomatic index (VSI) of NO and MO were significantly lower than FO (P0.05),NO组和MO组特定生长率、蛋白质效率和脏体比显著降低(P<0.05),饲料系数显著增大(P<0.05);MO组的全鱼粗蛋白含量显著高于其他组(P<0.05),FO组与MO组的全鱼粗脂肪含量显著高于其他组(P<0.05),FO组背肌粗灰分含量显著低于其他组(P<0.05);藻粉替代鱼油对肌肉和肝脏脂肪酸组分影响显著,肌肉的C16:0和DHA含量与其在饲料中所占的百分比呈显著正相关(r=0.973,0.967,P<0.05),C14:0和C16:1n-7呈极显著正相关(r=1.00,0.996,P<0.01),肝的C18:2n-6、n-6 PUFA和DHA/EPA呈显著正相关(r=0.983,0.976,0.977,P<0.05),C16:1n-7呈现极显著正相关(r=0.992,P<0.01);NO组和MO组血清谷丙转氨酶(ALT)、甘油三酯(TG)、胆固醇(CHO)和碱性磷酸酶(ALP)的含量较FO组显著降低(P<0.05),NO组血清谷草转氨酶(AST)和葡萄糖(GLU)含量显著低于其他组(P<0.05).研究表明,本实验条件下裂壶藻粉可以替代星斑川鲽幼鱼饲料中的鱼油而不对其生长和生理指标产生负面影响,并且在一定程度上能提高星斑川鲽的营养价值.
    • 李青; 郑风荣; 关洪斌; 倪世俊; 王波
    • 摘要: 本研究以线粒体基因细胞色素b (Cytochrome b,Cytb)和细胞色素C氧化酶Ⅰ(Cytochrome oxidase subunitⅠ,COI)为研究对象,探究比较了星斑川鲽(Platichthys stellatus)、石鲽(Kareius bicolorarus)以及杂交F1代(星斑川鲽♀×石鲽♂)的种质遗传特性.结果显示,杂交子代与亲本的碱基(A+T)含量均高于(C+G)含量,且杂交F1代与母本星斑川鲽的(C+G)相同.基于mtDNA Cytb和COI序列结果显示,石鲽与星斑川鲽遗传距离分别为0.085和0.045;杂交F1代与石鲽遗传距离分别为0.076和0.045.基于mtDNA Cytb序列显示,杂交F1代与星斑川鲽遗传距离很小,仅0.009,而两者在COI基因序列上完全一致.基于Kimura 2-parameter模型的NJ分子系统树均显示,星斑川鲽和杂交F1代聚为一支,石鲽单独聚为一支.以上结果均可得出,杂交后代在线粒体DNA上呈现明显的母系遗传.杂交后代中出现左右眼的分化,且在COI的NJ系统树中,杂交F1代与母本星斑川鲽形成的一大支又分为两支:母本星斑川鲽与杂交F1代中外观显示左眼的聚为一支,杂交F1代中外观显示右眼的单独聚为一小支.表明线粒体基因COI与杂交F1代左右眼的分化有一定关系,为进一步研究星斑川鲽♀x石鲽♂提供了参考数据.%Platichthys stellatus and Kareius bicoloratus are two common Pleuronectidae species that are highly valuable in the aquaculture industry.P.stellatus is endowed with high meat content and delicious taste while K.bicoloratus is highly resistant to diseases.Characteristics of their hybrids,however,were rarely reported.In this study,we explored the genetic variability of mtDNA sequences of P.stellatus,K.bicoloratus and their hybrid (P.stellatus♀ ×K.bicoloratus♂),using 24 P.stellatus,24 K.bicoloratus and 40 hybrids as samples.We found that that the content of A+T base pairs was higher than that of the C+G base pairs in all 3 groups,and that the content of A+T base pairs in the hybrids was the same as in P.stellatus.The analysis of mtDNA Cytb gene and COI fragments suggested that the genetic distances between K.bicoloratus and P.stellatus were 0.085 and 0.045 respectively,and that the genetic distances between the hybrids and K.bicoloratus were 0.076 and 0.045 separately.Moreover,there was a very small differentiation (0.009) between the hybrids and P.stellatus based on the mtDNA Cytb sequence analysis,and there was no genetic differentiation between the 2 groups based on the mtDNA COI gene sequence analysis.The neighbor-joining (NJ) phylogeny trees constructed with the Kimura 2-parameter model showed that P stellatus and the hybrid could be clustered into one branch,while K.bicoloratus belonged to another branch.In the NJ phylogenetic tree based on gene COI,the big branch including F1 hybrid and P.stellatus could be further divided into two small branches.One was P.stellatus and F1 hybrid with the left eyes,and the other was the F1 hybrid with the right eyes.These data strongly suggested that characteristics of the hybrid of P.stellatus and K.bicoloratus (P.stellatus ♀ x K.bicoloratus♂) were maternally inherited.This study provided helpful information for the future research on the hybridization of the two species.
    • 徐中平
    • 摘要: 该文研究了星斑川鲽无水保活运输技术方法及在无水保活处理中的应激反应.结果表明,星斑川鲽在低温麻醉状态下空气暴露处理65 h存活率为100%,处理84 h存活率达90%;血清皮质醇浓度在0~35 h和48~65 h时间段分别升高2~3倍和20-24倍,显示星斑川鲽的应激反应程度随胁迫进程呈现为阶梯状特点;血糖浓度变化趋势基本与血清皮质醇水平变化相对应.以上结果显示,星斑川鲽进行无水保活运输在该实验条件下可安全跨时35 h,且有可能跨时65 h,并保持100%存活.
    • 刘钢; 马德利; 冯春明
    • 摘要: 2016年辽宁某室内养殖的星斑川鲽种鱼发生疾病,尾炳出现溃烂等现象.从患病处分离致病菌并纯化,进行PCR检测及药敏试验.结果表明致病菌为哈维氏弧菌,在患处涂抹利福平治疗效果良好.
    • 田永胜; 李祥孔; 段会敏; 李洪; 宋莉妮; 陈张帆; 唐江; 王波; 孙宗哲
    • 摘要: The starry flounder Platichthys stellatus is one of the superior flatfish species in China and occupies an important place in flatfish aquaculture.350 brood fish consisting of 4 cultural populations were collected from Shandong Peninsula (Rizhao,Jiaonan,Weihai and Penglai).The body length and weight of 100 male and female brood fish were measured.The relationship of the power function between body length and weight was fitted as y=0.034 2x2.843 6 (R2 =0.565)(female)and y=0.075 9x2.533 4 (R2 =0.753 8)(male).55 male and 46 female fish were used for artificial reproduction with 4.39 times spawning and 2.36 times spermatogenesis.There were 25 successful cross combinations,which reproduced 29 half-sib families and full-sib families.After 150-day culture,the weight,full length,body length and width of 1 640 fries were measured.Variance components were evaluated by u-sing the minimum norm quadratic unbiased estimator (MINQUE).Heritability of growth-related traits was esti-mated by using additive-dominance model.Heritability (additive and dominant genetic effects)was predicted by u-sing linear unbiased prediction (LUP).Results showed that the genetic correlations among four traits were 0.809-0.999 (P <0.01)and variance components of their additive and dominant genetic effects were significantly dif-ferent (P <0.01).Narrow heritability was 0.311-0.444(P <0.01),while broad heritability was 0.377-0.525 (P <0.01).Additive genetic effects analysis showed that 6 of 31 parents were extremely significantly positive in these 4 traits (P <0.01),while 10 parents were extremely significantly (P <0.01)or significantly negative (P <0.05).Dominant random effects analysis showed that 6 of 25 cross combinations were extremely significantly posi-tive (P <0.01),while 8 were extremely significantly negative (P <0.01).All these results first provided abundant genetic data for the selection of good parents and cross combinations in the family lines establishment and seed cul-ture of the starry flounder.%星斑川鲽是优良的鲆鲽鱼类种质之一,在鲆鲽鱼类养殖产业中具有重要的地位。本研究初次收集了分布于山东半岛的4个养殖群体(日照、胶南、威海和蓬莱)的350尾亲鱼,分别测量了100尾雌雄亲鱼的体长和体质量,拟合了雌雄性亲鱼体质量和体长幂函数关系,分别为:y =0.0342x2.8436(R2=0.565)和 y=0.0759x2.5334(R2=0.7538)。利用其中成熟的55尾雄鱼和46尾雌鱼进行人工繁殖,雌鱼平均产卵次数为4.39次、雄鱼产精次数为2.36次。其中25个杂交组合繁殖成功,建立了29个半同胞和全同胞家系。在家系生长到150 d 时,对1640尾鱼苗体质量、全长、体长和体宽4个性状进行测量。利用最小范数二次无偏估计法(MINQUE)对以上性状的方差组分进行估计,利用“加-显性”模型对生长性状遗传力进行估计,采用线性无偏预测法(Linear Unbiased Prediction,LUP)预测亲本的加性和显性遗传效应。结果显示:4个性状的遗传相关系数为0.809~0.999(P <0.01)。体质量、全长、体长和体宽4个性状的加性、显性方差分量都达到极显著水平(P <0.01),狭义遗传率为0.311~0.444(P <0.01),广义遗传率为0.377~0.525(P <0.01)。加性遗传效应预测显示:31个亲本中的6个在4个性状上同时具有极显著的正向效应(P <0.01),10个亲本具有极显著(P <0.01)或显著(P<0.05)的负向效应。显性随机效应预测显示:25个杂交组合中6个具极显著正向效应(P <0.01),8个具有极显著负向效应(P <0.01)。研究结果首次为星斑川鲽家系建立和优良苗种培育筛选出了优良的亲本和杂交组合,同时为星斑川鲽的选择育种提供了丰富的遗传指标。
    • 郑风荣; 郭湘云; 刘洪展; 李青; 王波
    • 摘要: 为了研究星斑川鲽MHCⅡ类分子的作用及调控机制,实验通过SMART-RACE技术克隆得到了星斑川鲽MHCⅡ恒定链(MHCⅡIi)的全长cDNA序列,其长度为1766 bp,包含135 bp的5 '非编码区、837 bp的开放阅读框和794 bp的3'非编码区.该基因共编码279个氨基酸.理论分子量为30.848ku,等电点为6.89.与已知物种MHCⅡIi进行同源性比对,结果与狼鲈、紫红笛鲷和鳜关系较近,同源性均为79%.利用quantitative real-time PCR (qRT-PCR)技术检测了MHCⅡIi在星斑川鲽不同组织中的表达,以及爱德华氏菌感染前后对该基因在不同组织中表达水平的影响,结果显示:在脾脏、头肾、肝脏、后肠、性腺、心脏、血液、鳃和肌肉组织中,MHCⅡIi mRNA均有表达,但在表达量上有明显差异,脾脏和头肾组织相对表达水平较高,鳃、血液、肌肉、心脏和性腺中的表达水平较低.病原感染后,免疫相关组织脾脏和头肾的表达水平升高最明显,肝脏和后肠的表达水平也略有升高,但变化不明显.本研究可为星斑川鲽MHCⅡ类分子的作用机理提供理论依据,同时为海水养殖鱼类的抗病遗传育种工作提供研究基础.
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