摘要：
This study aimed to provide theoretical basis for clarifying velvet antler biological characteristics by comparative proteomics.In this study,different parts of Cervus elaphus songaricus velvet antler (on 65 and 75 days old) were studied by two-dimensional gel electrophoresis,MAL-DI-TOF-MS mass spectrometry identification and bioinformatics analysis.65 differentially expressed proteins were successfully identified,and these proteins were involved in the cellular process,developmental process,localization,metabolic process,response to stimulus,and the KEGG pathway of platelet activation,focal adhesion,metabolic pathways,PI3K-Akt signaling pathway.These proteins were associated with bone development (4 proteins),nerve development (3 proteins),vascular development (2 proteins),anti-oxidation and endoplasmic reticulum stress (4 proteins),immune (7 proteins),apoptosis (5 proteins).The results indicated that TTR,RBP4 and CRABP1 played important roles in regulating the growth and development of velvet antler in the process of antler's transition from 65 d to 75 d velvet antler.PRDX2,TXNDC5,ERP29,Pdia6 played important roles in endoplasmic reticulum stress and anti-oxidation.Endoplasmic reticulum stress,endoplasmic reticulum-related death pathway and mitochondrial apoptotic pathways could explain the rapid differentiation of velvet antler cells while a large amount of apoptosis,which could meet the rapid growth and development needs of velvet antler.In addition,GMFβ and CATHL1 may play important roles in nerve development and anti-inflammatory.%旨在从差异蛋白质组学角度为鹿茸生物学特性的阐明奠定理论基础.本研究以天山马鹿65和75 d鹿茸不同部位为试验材料,通过双向凝胶电泳、MALDI-TOF-MS质谱鉴定技术,结合生物信息学分析,成功鉴定出65种差异蛋白质,涉及代谢过程、细胞过程、发育过程、定位以及应激等生物学过程,还参与了血小板活化、黏着斑、代谢通路、PI3K Akt信号通路等代谢通路.这些蛋白包括骨发育相关蛋白4种、神经发育相关蛋白3种、血管发育相关蛋白2种、抗氧化、内质网应激相关蛋白4种、免疫相关蛋白7种、凋亡相关蛋白5种.结果显示,TTR、RBP4、CRABP1在由65 d鹿茸过渡到75 d鹿茸的过程中,对鹿茸生长发育起一定调控作用.PRDX2、TXNDC5、ERP29、Pdia6在内质网应激、抗氧化中起重要作用.内质网应激、内质网相关性死亡途径、线粒体凋亡途径可在一定程度上阐明鹿茸细胞快速增殖分化的同时存在大量细胞凋亡现象,以满足快速生长、发育的需要.另外,GMFβ、CATHL1两种蛋白在神经发育和炎症抗性中可能具有重要的作用.
