prognosis

摘要： Objective Many studies have revealed the crucial roles of miRNA in multiple human cancers,including lung adenocarcinoma(LUAD).In this study,we sought to explore new miRNA-mRNA pairs that are associated with LUAD prognosis.Methods A novel miRNA-mRNA regulatory network associated with prognosis in LUAD was identified and validated using the bioinformatic tools including OncomiR database,StarBase,miRnet,GEPIA2,UALCAN.Results Twenty key miRNAs were compiled after the analysis of the expression and prognostic value in OncomiR and StarBase.Targeted mRNAs of these key miRNAs were predicted in miRnet,and the resulting mRNAs were also analyzed for their prognostic values and expression patterns in GEPIA2 and UALCAN,respectively.Further expression correlation analysis was performed in StarBase.Subsequently,a new miRNA-mRNA network was built,of which each RNA pair showed negative expression correlation,opposite expression pattern,and prognostic value.Protein-protein interaction network was under construction for the mRNAs,and 19 hub genes were determined.Enrichment analysis showed that“Cell Cycle,Mitotic”was the most significantly enriched term.Then,a miRNA-hub gene sub-network was built.We selected and validated the regulatory relationship of some miRNA-hub pairs,including hsa-miR-1976/RFC2,hsa-let-7c-5p/RFC2,hsa-let-7c-5p/ESPL1,hsa-let-7c-5p/CDC25A,and hsa-miR-101-3p/KIF2C.Moreover,over-expression of hsa-miR-1976 and hsa-let-7c-5p resulted in significant cell cycle arrest.Conclusions Our results determined new prognosis-associated miRNA-mRNA pairs and might shed further light on the mechanism via which miRNA-mRNA network influences prognosis in LUAD.

摘要： The pathogenesis of temporal lobe epilepsy(TLE)was originally considered to be acquired.However,some reports showed that TLE was clustered in some families,indicating a genetic etiology.With the popularity of genetic testing technology,eleven different types of familial TLE(FTLE),including ETL1-ETL11,have been reported,of which ETL9-ETL11 had not yet been included in the OMIM database.These types of FTLE were caused by different genes/Loci and had distinct characteristics.ETL1,ETL7 and ETL10 were characterized by auditory,visual and aphasia seizures,leading to the diagnosis of familial lateral TLE.ETL2,ETL3 and ETL6 showed prominent autonomic symptom and automatism with or without hippocampal abnormalities,indicating a mesial temporal origin.Febrile seizures were common in FTLEs such as ETL2,ETL5,ETL6 and ETL11.ETL4 was diagnosed as occipitotemporal lobe epilepsy with a high incidence of migraine and visual aura.Considering the diversity and complexity of the symptoms of TLE,neurologists enquiring about the family history of epilepsy should ask whether the relatives of the proband had experienced unnoticeable seizures and whether there is a family history of other neurological diseases carefully.Most FTLE patients had a good prognosis with or without anti-seizure medication treatment,with the exception of patients with heterozygous mutations of the CPA6 gene.The pathogenic mechanism was diverse among these genes and spans disturbances of neuron development,differentiation and synaptic signaling.In this article,we describe the research progress on eleven different types of FTLE.The precise molecular typing of FTLE would facilitate the diagnosis and treatment of FTLE and genetic counseling for this disorder.

摘要： Third trimester bleeding is a common concern in obstetrics. The main objective of this work was to study the management of hemorrhages in the third trimester of pregnancy in the maternity ward of the Sominé Dolo hospital in Mopti. Our prospective descriptive cross-sectional survey type study conducted at the maternity ward of Sominé Dolo hospital in Mopti over a period from January 1, 2017 to December 31, 2017 included 94 cases collected. During this period we had performed 1485 deliveries including 94 cases of pregnancies complicated by 3rd trimester hemorrhage, a frequency of 6.33%. The main cause of hemorrhage in the third trimester was represented by placenta preavia 42.6% followed by retroplacental hematoma 28.7%, uterine rupture 26.6% and association Placenta preavia and retroplacental hematoma 2.1%. The type of intervention depended on the cause of the hemorrhage and the maternal and fetal condition. More than half of the cases of uterine rupture 52% had benefited from a hysterorrhaphy during a laparotomy (n = 13/25) against 48% from hysterectomy (n = 12/25). Caesarean section was performed in 87.5% (n = 35/40) against 12.5% vaginal delivery (n = 5/40) in case of placenta preavia. In the end, in 74% of cases (n = 20/27) of retroplacental hematoma, first-line cesarean section was performed. The maternal prognosis was represented by a mortality rate of 12% (n = 11/94) and morbidity dominated by hypovolemic shock 48.9% (n = 22/94), infections 28.8% (n = 13/94) and coagulopathy 11.1% (n = 5/94). The fetal prognosis was very poor. More than half (55%) of the newborns had succumbed against 45% of the newly born. In 55.3% of cases neonatal mortality occurred antenatally. Neonatal morbidity was represented by prematurity, i.e. 20.2% (n = 19/94) and low birth weight, i.e. 22.3% (n = 21/94).

摘要： BACKGROUND The N-Myc downstream-regulated gene(NDRG)family is comprised of four members(NDRG1-4)involved in various important biological processes.However,there is no systematic evaluation of the prognostic of the NDRG family in hepatocellular carcinoma(HCC).AIM To analyze comprehensively the biological role of the NDRG family in HCC.METHODS The NDRG family expression was explored using The Cancer Genome Atlas.DNA methylation interactive visualization database was used for methylation analysis of the NDRG family.The NDRG family genomic alteration was assessed using the cBioPortal.Single-sample Gene Set Enrichment Analysis was used to determine the degree of immune cell infiltration in tumors.RESULTS NDRG1 and NDRG3 were up-regulated in HCC,while NDRG2 was down-regulated.Consistent with expression patterns,high expression of NDRG1 and NDRG3 was associated with poor survival outcomes(P<0.05).High expression of NDRG2 was associated with favorable survival(P<0.005).An NDRG-based signature that statistically stratified the prognosis of the patients was constructed.The percentage of genetic alterations in the NDRG family varied from 0.3%to 11.0%,and the NDRG1 mutation rate was the highest.NDRG 1-3 expression was associated with various types of infiltrated immune cells.Gene ontology analysis revealed that organic acid catabolism was the most important biological process related to the NDRG family.Gene Set Enrichment Analysis showed that metabolic,proliferation,and immune-related gene sets were enriched during NDRG1 and NDRG3 high expression and NDRG2 low expression.CONCLUSION Overexpression of NDRG1 and NDRG3 and down-expression of NDRG2 are correlated with poor overall HCC prognosis.Our results may provide new insights into the indispensable role of NDRG1,2,and 3 in the development of HCC and guide a promising new strategy for treating HCC.

摘要： BACKGROUND In recent years,the predictive role of YKL-40 for long-term survival in colorectal cancer patients has been gradually investigated.However,whether it is a reliable and valuable prognostic indicator for patients with colorectal carcinoma has not been verified.AIM To identify the prognostic value of serum/plasma concentration of YKL-40 or expression status of YKL-40 in tumor cells in colorectal carcinoma patients.METHODS Several electronic databases including the PubMed,EMBASE,Web of Science,CNKI,VIP and WanFang were searched for relevant studies.The hazard ratios(HR)and 95%confidence intervals(CI)were combined and the primary and secondary outcomes were overall survival(OS)and progression-free survival(PFS),respectively.All statistical analysis were conducted by STATA 15.0 software.RESULTS A total of nine studies involving 2545 patients were included.The pooled results indicated that YKL-40 was significantly associated with poor OS(HR=1.80,95%CI:1.32-2.45,P<0.001)and PFS(HR=1.62,95%CI:1.22-2.16,P=0.001).Subgroup analysis stratified by the treatment,tumor type and source of YKL-40 showed similar results.CONCLUSION Elevated serum/plasma concentration of YKL-40 or positive expression in tumor cells was related with worse prognosis of colorectal carcinoma patients.YKL-40 might serve as a novel and reliable indicator for the evaluation of prognosis in colorectal cancer.

摘要： BACKGROUND Some studies investigated the prognostic role of several blood biomarkers,including the neutrophil/lymphocyte ratio(NLR),platelet/lymphocyte ratio(PLR),lymphocyte/monocyte ratio(LMR)and Glasgow prognostic score(GPS),in osteosarcoma,but their results were inconsistent with each other.AIM To identify the prognostic value of NLR,PLR,LMR and GPS in osteosarcoma patients through reviewing relevant studies.METHODS The PubMed,EMBASE,Web of Science and CNKI databases were searched up to October 2,2021.The primary and second outcomes were overall survival(OS)and disease-free survival(DFS),respectively.The hazard ratios(HRs)with 95%confidence intervals(CIs)were combined to assess the association between these indicators and prognosis of osteosarcoma patients.RESULTS A total of 13 studies involving 2087 patients were eventually included.The pooled results demonstrated that higher NLR and GPS were significantly associated with poorer OS(HR=1.88,95%CI:1.38-2.55,P<0.001;HR=2.19,95%CI:1.64-2.94,P<0.001)and DFS(HR=1.67,95%CI:1.37-2.04,P<0.001;HR=2.50,95%CI:1.39-4.48,P<0.001).However,no significant relationship of PLR and LMR and OS(P=0.085;P=0.338)and DFS(P=0.396;P=0.124)was observed.CONCLUSION Higher NLR and GPS were related with worse prognosis and might serve as novel prognostic indicators for osteosarcoma patients.

摘要： Introduction: The artificial induction of labor is increasingly common. Objective: Evaluate the maternal-fetal prognosis of artificial induction with misoprostol at the referral health center of the commune II. Methods: This is a cross-sectional, prospective, descriptive and analytical study which took place from September 1, 2019 to December 31, 2020. Included in the study were pregnant women carrying an evolving or terminated single-fetal pregnancy of at least 28 WA in cephalic presentation on a healthy uterus. The trigger has been made with 50 μg misoprostol administered sublingually into the posterior vaginal fornix. The dose was renewed as needed every 6 hours, until sufficient uterine contractions were obtained without exceeding 200 μg. Results: The frequency of artificial induction of labor was 1.25%. Indications were dominated by premature rupture of membranes (29.8%), overdue (19.1%), high blood pressure (19.1%), suitability of pregnant women (14.9%) and terminated pregnancies (10.6%). The vaginal delivery rate was 85.1% The Apgar score was greater than or equal to 7 in 83.3% of cases at the 1stminute. Maternal morbidity was marked by postpartum hemorrhage due to uterine atony in 4.3% cases. No maternal and neonatal deaths were noted. Conclusion: Induction of labor with misoprostol is a safe and effective method if careful selection of patients is made.

摘要： BACKGROUND Breast cancer mainly occurs in young and premenopausal women;its incidence is increasing annually. Patients with triple-negative breast cancer(TNBC) have relatively high recurrence and transfer rates during the operation and 3 years after postoperative adjuvant chemotherapy. Currently, the treatment for patients with TNBC is mainly based on a comprehensive combination of surgery and chemotherapy. Therefore, identifying additional effective treatments to improve patient prognosis is important.AIM To explore and discuss the effects and prognostic factors of neoadjuvant chemotherapy in TNBC.METHODS In total, 118 patients diagnosed with TNBC from January 2016 to January 2020 in our hospital were selected and divided into the observation(n = 60) and control(n = 58) groups according to therapeutic regimen. The control group received routine chemotherapy, and the observation group received neoadjuvant chemotherapy. The therapeutic effects of the two groups were observed, and the survival of patients was followed up.RESULTS The karyopherin A2(KPNA2)-positive and SRY-related HMG box-2(SOX2)-positive expression rates of patients with TNBC with intravascular tumor thrombus and tumor-node-metastasis(TNM) stage IV were 92.00% and 91.67% and 96.00% and 95.83%, respectively, which were significantly higher than those of patients with no intravascular tumor thrombus and TNM stage Ⅲ(P < 0.05). KPNA2 was positively associated with SOX2 expression(rs = 0.514, P < 0.50). The short-term curative effect of the observation group was better than that of the control group(P < 0.05), and the total effective rate was 58.33%. After treatment, carcinoembryonic antigen, cancer antigen(CA) 19-9, and CA125 Levels in the observation group were 11.40 ± 2.32 mg/L, 19.92 ± 3.42 kU/L, and 54.30 ± 12.28 kU/L, respectively, which were significantly lower than those in the control group(P < 0.05). The median survival time of the observation group was 33 mo(95%CI: 31.21-34.79), which was significantly longer than that of the control group(P < 0.05). TNM stage, degree of differentiation, lymph node metastasis, KPNA2 and SOX2 expressions, and treatment plan were prognostic factors of TNBC(relative risk = 1.575, 1.380, 1.366, 1.433, 1.411, and 0.581, respectively, P < 0.05).CONCLUSION Neoadjuvant chemotherapy for TNBC treatment can achieve good curative effects. TNM stage, differentiation degree, lymph node metastasis, KPNA2 and SOX2 expressions, and treatment plan are prognostic factors of TNBC.

摘要： BACKGROUND Hepatic resection has become the preferred treatment of choice for colorectal liver metastasis(CLM)patients.AIM To identify the prognostic factors and to formulate a new scoring system for management of CLM.METHODS Clinicopathologic and long-term survival data were analyzed to identify the significant predictors of survival by univariate and multivariate analyses with the Cox model.A clinical score was constructed based on the analysis results.RESULTS Three factors of worse overall survival were identified in the multivariate analysis.They were number of liver metastases≥5,size of the largest liver lesion≥4 cm,and the presence of nodal metastasis from the primary tumor.These three factors were chosen as criteria for a clinical risk score for overall survival.The clinical score highly correlated with median overall survival and 5-year survival(P=0.002).CONCLUSION Priority over surgical resection should be given to the lowest score groups,and alternative oncological treatment should be considered in patients with the highest score.

摘要： BACKGROUND In China,it has been well recognized that some female patients with esophageal squamous cell carcinoma(ESCC)have different overall survival(OS)time,even with the same tumor-node-metastasis(TNM)stage,challenging the prognostic value of the TNM system alone.An effective predictive model is needed to accurately evaluate the prognosis of female ESCC patients.AIM To construct a novel prognostic model with clinical and reproductive data for Chinese female patients with ESCC,and to assess the incremental prognostic value of the full model compared with the clinical model and TNM stage.METHODS A new prognostic nomogram incorporating clinical and reproductive features was constructed based on univariatie and Cox proportional hazards survival analysis from a training cohort(n=175).The results were recognized using the internal(n=111)and independent external(n=85)validation cohorts.The capability of the clinical–reproductive model was evaluated by Harrell’s concordance index(C-index),Kaplan–Meier curve,time-dependent receiver operating characteristic(ROC),calibration curve and decision curve analysis.The correlations between estrogen response and immune-related pathways and some gene markers of immune cells were analyzed using the TIMER 2.0 database.RESULTS A clinical–reproductive model including incidence area,age,tumor differentiation,lymph node metastasis(N)stage,estrogen receptor alpha(ESR1)and beta(ESR2)expression,menopausal age,and pregnancy number was constructed to predict OS in female ESCC patients.Compared to the clinical model and TNM stage,the time-dependent ROC and C-index of the clinical–reproductive model showed a good discriminative ability for predicting 1-,3-,and 5-years OS in the primary training,internal and external validation sets.Based on the optimal cut-off value of total prognostic scores,patients were classified into high-and low-risk groups with significantly different OS.The estrogen response was significantly associated with p53 and apoptosis pathways in esophageal cancer.CONCLUSION The clinical–reproductive prognostic nomogram has an incremental prognostic value compared with the clinical model and TNM stage in predicting OS in Chinese female ESCC patients.

摘要： Rotator cuff tear is one of the most common disorders of the shoulder. To examine the prognostic indicators associated with clinical outcome in rotator cuff surgery, a retrospective evaluation of 28 multivariate factors in 1,120 shoulders in 1,067 patients was performed. The data was analyzed by logistic regression and Kendall's tau-b correlation analysis. The degree of degenerative changes correlated with several factors that were selected for further evaluation using logistic regression by forward stepwise analysis. The results show that there was a high correlation with age, degree and type of tendon tear, preoperative pain and preoperative muscle power. The correlation between the type of tear, surgical outcome and the other factors in RCT was analyzed by nonparametric correlation methods. High correlation was seen between the type of tear and the tendons involved, degree of retraction, age, subacromial bone spur, operative technique,pre-and post-operative muscle power, pre-operative abduction and hand dominance. Additionally, there was a high correlation between good post-operative outcome and post-operative activities of daily living, post-operative pain, post-operative muscle power, degree of tendon retraction, pre-operative activities of daily living, type of tendon tear, pre-operative external rotation, muscle power and pain, and duration of symptoms.

摘要： Rotator cuff tear is one of the most common disorders of the shoulder. To examine the prognostic indicators associated with clinical outcome in rotator cuff surgery, a retrospective evaluation of 28 multivariate factors in 1,120 shoulders in 1,067 patients was performed. The data was analyzed by logistic regression and Kendall's tau-b correlation analysis. The degree of degenerative changes correlated with several factors that were selected for further evaluation using logistic regression by forward stepwise analysis. The results show that there was a high correlation with age, degree and type of tendon tear, preoperative pain and preoperative muscle power. The correlation between the type of tear, surgical outcome and the other factors in RCT was analyzed by nonparametric correlation methods. High correlation was seen between the type of tear and the tendons involved, degree of retraction, age, subacromial bone spur, operative technique,pre-and post-operative muscle power, pre-operative abduction and hand dominance. Additionally, there was a high correlation between good post-operative outcome and post-operative activities of daily living, post-operative pain, post-operative muscle power, degree of tendon retraction, pre-operative activities of daily living, type of tendon tear, pre-operative external rotation, muscle power and pain, and duration of symptoms.

摘要： Rotator cuff tear is one of the most common disorders of the shoulder. To examine the prognostic indicators associated with clinical outcome in rotator cuff surgery, a retrospective evaluation of 28 multivariate factors in 1,120 shoulders in 1,067 patients was performed. The data was analyzed by logistic regression and Kendall's tau-b correlation analysis. The degree of degenerative changes correlated with several factors that were selected for further evaluation using logistic regression by forward stepwise analysis. The results show that there was a high correlation with age, degree and type of tendon tear, preoperative pain and preoperative muscle power. The correlation between the type of tear, surgical outcome and the other factors in RCT was analyzed by nonparametric correlation methods. High correlation was seen between the type of tear and the tendons involved, degree of retraction, age, subacromial bone spur, operative technique,pre-and post-operative muscle power, pre-operative abduction and hand dominance. Additionally, there was a high correlation between good post-operative outcome and post-operative activities of daily living, post-operative pain, post-operative muscle power, degree of tendon retraction, pre-operative activities of daily living, type of tendon tear, pre-operative external rotation, muscle power and pain, and duration of symptoms.

摘要： Rotator cuff tear is one of the most common disorders of the shoulder. To examine the prognostic indicators associated with clinical outcome in rotator cuff surgery, a retrospective evaluation of 28 multivariate factors in 1,120 shoulders in 1,067 patients was performed. The data was analyzed by logistic regression and Kendall's tau-b correlation analysis. The degree of degenerative changes correlated with several factors that were selected for further evaluation using logistic regression by forward stepwise analysis. The results show that there was a high correlation with age, degree and type of tendon tear, preoperative pain and preoperative muscle power. The correlation between the type of tear, surgical outcome and the other factors in RCT was analyzed by nonparametric correlation methods. High correlation was seen between the type of tear and the tendons involved, degree of retraction, age, subacromial bone spur, operative technique,pre-and post-operative muscle power, pre-operative abduction and hand dominance. Additionally, there was a high correlation between good post-operative outcome and post-operative activities of daily living, post-operative pain, post-operative muscle power, degree of tendon retraction, pre-operative activities of daily living, type of tendon tear, pre-operative external rotation, muscle power and pain, and duration of symptoms.

摘要： Rotator cuff tear is one of the most common disorders of the shoulder. To examine the prognostic indicators associated with clinical outcome in rotator cuff surgery, a retrospective evaluation of 28 multivariate factors in 1,120 shoulders in 1,067 patients was performed. The data was analyzed by logistic regression and Kendall's tau-b correlation analysis. The degree of degenerative changes correlated with several factors that were selected for further evaluation using logistic regression by forward stepwise analysis. The results show that there was a high correlation with age, degree and type of tendon tear, preoperative pain and preoperative muscle power. The correlation between the type of tear, surgical outcome and the other factors in RCT was analyzed by nonparametric correlation methods. High correlation was seen between the type of tear and the tendons involved, degree of retraction, age, subacromial bone spur, operative technique,pre-and post-operative muscle power, pre-operative abduction and hand dominance. Additionally, there was a high correlation between good post-operative outcome and post-operative activities of daily living, post-operative pain, post-operative muscle power, degree of tendon retraction, pre-operative activities of daily living, type of tendon tear, pre-operative external rotation, muscle power and pain, and duration of symptoms.