Closed culture for enhanced culture and gene editing of tumor infiltrating lymphocytes and its use in immunotherapy

摘要

The present invention provides an enhanced efficacy of TIL, improved phenotype and increased metabolic health in a shorter period of time, while expanding the IL, including a novel method for expanding cultures of TIL populations in the closed system, enabling reduced microbial contamination and reduced costs; An improved and / or shortened method for producing a therapeutic IL population may be provided.The method may include gene editing at least a portion of the til to enhance its therapeutic efficacy.Such tiil finds use in therapeutic regimen.