EXTINCTION DILUTION CULTURE GROUP-BASED HIGH-THROUGHPUT ANALYSIS AND BREEDING METHOD FOR FUNCTIONAL MICROORGANISMS

摘要

Provided is an extinction dilution culture group-based high-throughput analysis and breeding method for functional microorganisms. According to the invention, microbial communities with different functional activity characteristics can be enriched from environmental samples, particularly from complex environmental systems such as soil and sediments, by means of gradient dilution under different dilution degrees; and high-throughput sequencing is carried out on the communities, and the structural composition thereof is analyzed. According to the composition characteristics of the communities, aiming at different types of appearing microorganisms, an isolation culture medium is designed for separation and screening. According to the method, a species with a high relative abundance can be obtained, and rare species with a low relative abundance can also be separated.