NON-DIAGNOSTIC METHOD FOR DETECTING PD-L1 GENE MUTATION OF PATIENT WITH COLORECTAL CANCER BY MEANS OF CIRCULATING TUMOR CELLS IN PERIPHERAL BLOOD

摘要

Provided in the present invention is a non-diagnostic method for detecting a PD-L1 gene mutation of a patient with colorectal cancer by means of circulating tumor cells in peripheral blood, which belongs to the field of molecular biology. The detection method mainly comprises collecting peripheral blood, treating the peripheral blood, filtering and enriching circulating tumor cells, and using an immunohistochemical method to detect the PD-L1 gene expression of the circulating tumor cells. The detection method provided by the present invention can be used to detect the PD-L1 expression of a patient with colorectal cancer without obtaining a tissue specimen by means of needle biopsy. The technique is minimally invasive and can realize real-time detection.Moreover, the method can avoid a false positive result caused by an edge effect which may be generated in a dyeing process, has good stability, reduces cell loss, and improves detection accuracy.