DORSAL AORTIC INJECTION METHOD OF CHICKEN EMBRYOS FOR IMPROVING TRANSGENIC EFFICIENCY

摘要

A dorsal aortic injection method of chicken embryos for improving transgenic efficiency, comprising the following steps: injecting adeno-associated virus particles into the dorsal aorta of each 2.5-day-old chicken embryo outside eggshell, individually transferring the injected chicken embryos into their recipient eggshells (coming from double yolk eggs), and proceeding to hatch. The disclosure realizes the outside-eggshell injection of the chicken embryo dorsal aorta through the modified dorsal aortic injection method, improves the accuracy of injection, and also realizes the direct observation of the development process of the chicken embryos. The survival rate of embryos, hatch rate and the efficiency of transgene expression in the modified dorsal aortic injection group are significantly higher than those in the subgerminal cavity injection group and the classic dorsal aortic injection group, indicating that the modified method improves the hatch rate of transgenic chicken embryos and EGFP expression efficiency.