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METHODS FOR DYNAMIC EVOLUTION, ADAPTATION, AND MONITORING OF CHARACTERISTICS IN LIVING CELLS USING A MICROFLUIDIC-ENABLED MULTI-WELL CELL CULTURE DEVICES AND SYSTEMS
METHODS FOR DYNAMIC EVOLUTION, ADAPTATION, AND MONITORING OF CHARACTERISTICS IN LIVING CELLS USING A MICROFLUIDIC-ENABLED MULTI-WELL CELL CULTURE DEVICES AND SYSTEMS
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机译:使用能够进行微流体的多孔细胞培养装置和系统的动态演化,适应和监测活细胞特征的方法
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摘要
A method for dynamic evolution and/or adaptation and monitoring of characteristics in living cells is provided, wherein the method may be performed at a microfluidic-enabled cell-culture device comprising pneumatic layer for directing flow of fluid to a plurality of individually addressable wells, and one or more sensors configured to detect data regarding environments inside one or more of the plurality of wells. The method may involve culturing a population of cells in a first well of the plurality of wells, perturbing one or more characteristics of an environment in the first well following the culturing of the population of cells, monitoring one or more characteristics of the population of cells in the first well, and removing all or part of the evolved/adapted population of cells from the first well.
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