IMMUNE COMBINATION FOR INDUCING BROAD-SPECTRUM NEUTRALIZING ANTIBODY AGAINST HIV-1

摘要

The present invention relates to the field of vaccines, and relates in particular to a vaccine combination for inducing a broad-spectrum neutralizing antibody against HIV-1. The form of a combined vaccine is employed, an HIV-1 membrane protein is used as an immunogen in a first injection; vaccine presentation forms comprise but are not limited to a recombinant plasmid vaccine, a recombinant protein subunit vaccine, a recombinant virus vector vaccine, a virus-like particle vaccine, a nanoparticle vaccine, an attenuated live vaccine and an inactivated virus vaccine; a second injection employs a virus vector vaccine for expressing an HIV-1 membrane protein, which can substantially improve the titer and broad spectrum properties of an induced anti-HIV-1 neutralizing antibody. The virus vector comprises but is not limited to poxvirus, adenovirus, herpes simplex virus, measles virus, enterovirus and rhabdovirus. A third injection and fourth injection are protein nanoparticle vaccine and virus vector vaccine combinations or vice versa. Inoculation modes of the vaccine comprise but are not limited to muscle inoculation, intracutaneous inoculation, subcutaneous inoculation, nasal drops, aerosol inhalation, the genital tract, the rectum, oral administration, and so on. The results of animal experiments prove that the vaccine combination is safe, can continuously generate a high-titer broad-spectrum neutralizing antibody, and can be used for preventing and treating AIDS in the future.