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DETERMINATION OF THE DEGREE OF DISSOCIATION OF AN A-I APOPROTEIN AND METHOD FOR PREDICTING THE STRUCTURAL AND FUNCTIONAL PROPERTIES OF HIGH-DENSITY LIPOPROTEINS OF HUMAN BLOOD PLASMA CONTROLLING THE MAIN ROUTE OF RELEASE OF CHOLESTEROL FROM MACROPHAGES
DETERMINATION OF THE DEGREE OF DISSOCIATION OF AN A-I APOPROTEIN AND METHOD FOR PREDICTING THE STRUCTURAL AND FUNCTIONAL PROPERTIES OF HIGH-DENSITY LIPOPROTEINS OF HUMAN BLOOD PLASMA CONTROLLING THE MAIN ROUTE OF RELEASE OF CHOLESTEROL FROM MACROPHAGES
FIELD: medicine.;SUBSTANCE: invention relates to the field of lipoproteomics, metabolomics, cellular biology, atherogenesis, structural biology, molecular cardiology. The invention pertains to a method for determining the degree of dissociation of an A-I apoprotein. The method includes collecting blood in male patients without atherosclerosis and performing denaturation of high-density lipoprotein (HDL) preparations obtained by precipitation of apoB-containing lipoproteins with polyethylene glycol by urea; measuring the dissociation of apoA-I occurring during denaturation of the HDL by the accumulation of preβ-apoA-I in separation of the HDL by electrophoresis in an agarose gel followed by immunodetection of an apoprotein in a replica of the gel; the effect of induction of dissociation of apoA-I by urea on the functional properties of the HDL is identified by measuring the АВСА1-mediated efflux of a fluorescent cholesterol analogue from the mouse macrophage line RAW 264.7 on the HDL of patients as a cholesterol acceptor. The degree of dissociation of apoA-I (D) is then calculated as the gain in the proportion of preβ-apoA-I relative to the native HDL by the formula.;EFFECT: invention ensures identification of new structural and functional properties of high-density lipoprotein particles of human blood plasma determining the atheroprotective properties thereof.;1 cl, 3 tbl, 4 dwg
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