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MATURATION OF SPERMATOGONIAL CELLS IN VITRO BT GROWTH FACTORS AND HORMONES
MATURATION OF SPERMATOGONIAL CELLS IN VITRO BT GROWTH FACTORS AND HORMONES
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机译:在体外BT生长因子和激素的精术细胞成熟
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摘要
Provided are methods of in vitro maturation of spermatogonium by culturing the spermatogonium in a three-dimensional methylcellulose culture system (MCS) in a culture medium which comprises an effective concentration of a factor selected from the group consisting of granulocyte macrophages-colony stimulating factor (GM-CSF), interleukin- 1 alpha (IL-1 alpha), interleukin- 1 beta (IL-lbeta) and interleukin-6 (IL-6), under conditions capable of differentiating the spermatogonium into at least meiotic and/or postmeiotic cells, thereby in vitro maturing the spermatogonium.
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