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Bovine serum composition and method for culturing cells using said bovine serum composition as additive
Bovine serum composition and method for culturing cells using said bovine serum composition as additive
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机译:使用所述牛血清组合物作为添加剂的牛血清组合物和培养细胞的方法
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摘要
Provided is a method for producing an economical bovine serum composition containing many factors useful for cell proliferation. The method includes a step of performing an anticoagulation treatment of bovine whole blood with an anticoagulant, a step of obtaining a buffy coat and a fraction with a heavier specific gravity than that of the buffy coat from the anticoagulated whole blood, and a step of promoting and activating an interaction between the obtained leukocytes and platelets at a given temperature for not less than a given time to cause secretion or release of a humoral factor from the leukocytes and/or platelets and performing a recoagulation treatment of blood components including the humoral factor with a re-coagulating agent.
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