The method of in vitro differentiation and polarization of macrophages for improving or inhibiting infectivity against PRRSV

摘要

The present invention relates to a method for inducing differentiation from porcine peripheral blood mononuclear cells into M1 macrophages or M2 macrophages, a screening method for PRRSV proliferation enhancing substances using M1 macrophages differentiated by the method, and PRRSV proliferation inhibition using M2 macrophages A method for screening a substance for use is provided. Conventionally, there was inconvenience in that alveolar macrophages had to be directly collected from the lungs by killing pigs for PRRSV isolation and culture. However, using the method of the present invention is very useful in that M1 macrophages and M2 macrophages can be differentiated from porcine peripheral blood mononuclear cells obtained from swine blood, and these can be used for culturing, propagating and isolating PRRSV.