RAPD Random Amplified Polymorphic DNA primer for discrimination of tobacco cultivars

摘要

The present invention relates to a method for identifying a tobacco variety by RAPD (Random Amplified Polymorphic DNA) analysis and a primer used in the method, and more specifically, a specific base sequence selected from the group consisting of SEQ ID NOs: 1 to 14 It relates to a RAPD primer having a RAPD primer, a method for detecting DNA polymorphism in tobacco, and a method for identifying a tobacco variety, comprising performing PCR using the same. The RAPD marker for tobacco variety discrimination according to the present invention can be very usefully used to create a tobacco DNA profile by effectively detecting DNA polymorphisms in tobacco. It can effectively perform an analysis of redundancy with existing resources and establish novelty, and has the effect of discriminating the variety of tobacco. In addition, it is possible to clearly distinguish between native varieties (lineages) scattered in Korea, and it is possible to register new varieties of unnamed varieties to protect our precious genetic resources, and to distinguish between domestic and imported varieties. It can contribute to establishing the distribution order in the tobacco market.