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Screening method based on cell-free protein synthesis to determine the factors affecting the expression efficiency or activity of recombinant proteins
Screening method based on cell-free protein synthesis to determine the factors affecting the expression efficiency or activity of recombinant proteins
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机译:基于无细胞蛋白质合成的筛选方法确定影响重组蛋白表达效率或活性的因素
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摘要
The present invention relates to a screening method for a protein expression or activity enhancing cofactor using cell-free protein synthesis. Specifically, in the production of a recombinant protein, the expression of a protein and the formation of a functional three-dimensional structure are affected by various factors. , relates to a screening method that greatly improves the productivity of a target protein having activity by sequentially expressing a cofactor protein and a target protein that help in the synthesis and functional structure of a specific protein.
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