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AN EFFICIENT CRYOPRESERVATION DEVICE PREVENTING THE DIRECT CONTACT BETWEEN SAMPLES AND EXTRACELLUAR ICE
AN EFFICIENT CRYOPRESERVATION DEVICE PREVENTING THE DIRECT CONTACT BETWEEN SAMPLES AND EXTRACELLUAR ICE
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机译:一种有效的冷冻保存装置,防止样品和细胞外冰的直接接触
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摘要
A cryoprotective device protects an aqueous biological material from mechanical damage due to ice formation during cryogenic freezing and/or cryostorage by preventing direct contact of the biological material with cell-damaging large ice crystals, the cryoprotective storage device having a housing with an internal cavity. The housing is configured to receive a freezable medium with the biological material within the internal cavity. The housing includes a semi-permeable membrane. The membrane is impermeable to ice crystals that are larger than an average pore size of the membrane to prevent such ice crystals from passing into the internal cavity from outside the housing, such that ice crystals formed in the medium within the housing have a smaller crystal size from ice crystals formed in the medium outside of the housing. As such, the biological material is protected from mechanical damage generated by direct contact with large ice crystals.
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