NANOZYME LINKED OLIGO PROBE SORBENT ASSAY (NLOPSA) FOR THE DETECTION OF NUCLEIC ACID BIOMARKERS

摘要

The invention provides a nanozyme for detecting one or more nucleic acid biomarkers and method for preparation thereof. The nanozyme includes a metallic nanoparticle, and a plurality of single-stranded oligonucleotide probes with thiol modification at a 5' end thereof, wherein each of the single-stranded oligonucleotide probes are conjugated via thiol chemisorption on the nanoparticle surface. The present invention also discloses nanozyme linked oligo probe assay (NLOPSA) for detection of one or more nucleic acid biomarkers.