Method for measuring arginase activity, detection kit of arginase activity, arginase related disease detection kit, and method for screening inhibitor or activator of arginase

摘要

The present invention provides an easy and simple method for measuring arginase activity with high sensitivity. In the method for measuring arginase activity of the present invention, a sample containing arginase and a solution containing a divalent cation are added to a reaction vessel to activate arginase, and the sample containing activated arginase is added to the reaction vessel. Adding an arginine solution and performing an enzyme substrate reaction; and adding an acidic solution containing a urea detection reagent to a pH of 1.0 or more and 4.0 or less to the sample subjected to the enzyme substrate reaction; And performing the detection reaction at the same time.