CORNEAL EPITHELIAL CELLS AND THEIR PRODUCTS FOR TREATING CORNEAL DISEASES

摘要

The present invention includes compositions and method of generating human corneal epithelial stem cells, a human corneal epithelial stem cell supernatant, or both, the method comprising: wetting and mincing a corneal epithelial sample in a media, drying the minced corneal epithelial sample until sample edges are adhered to a substrate, adding a growth media comprising fetal bovine serum or equivalent to the minced corneal epithelial sample without dislodging the minced corneal epithelial sample from the substrate with an amount of media that permits at least a portion of the minced corneal epithelial sample to be in contact with air, culturing the minced corneal epithelial sample for one or more days, changing the growth media to a media comprising a human corneal growth supplement (HCGS) with no fetal bovine serum, culturing the cells to grow human corneal epithelial stem cells, a human corneal epithelial stem cell supernatant, or both.