Methods for establishing high expression cell line

摘要

The present invention relates to an expression vector for establishing a highly expressing cell line comprising an antibiotic selection marker gene expression cassette linked to an IRES and a protein destabilizing sequence, a transformed cell using the same, a method for establishing a highly expressing cell line, and a method for high production of a protein of interest. . According to the present invention, since only highly expressing cell lines can be quickly and easily selected while blocking the occurrence of false-positive cells, efficient production of recombinant proteins is possible.