A method to obtain the seedling of hyakusanagi sugi (Cryptomeria japonica D. Don) by embryo rescue technique

摘要

An object of the present invention is to disclose a method for obtaining seedlings of Hyakusanso cold cedar in a short time by embryo rescue technology, and to solve the problem that the Hyakusanso cold cedar is extremely threatened with extinction. In the present invention, the immature coniferous fruit of the wild Baishanzu cold cedar plant in the Baishanzu Nature Reserve is used as a starting material, the surface is disinfected, the immature embryo and endosperm are taken, and the embryo rescue technique is used. The descendants of the wild Hyakusanso cold cedar plant can be obtained in a short time, and many Hyakusanso cold cedar seedlings can be obtained in a short time. The present invention accelerates the growth rate of Hyakusanso cold cedar seedlings by culturing immature embryos of Hyakusanso cold cedar for 30 days to obtain many Hyakuyamaso cold cedar seedlings. It has the advantage of improving the current situation in which cold cedar is endangered under natural conditions. The method has important implications for the rapid growth of endangered plant seedlings in the world, with high operability, simplicity and efficiency, low contamination rates. [Selection diagram] Fig. 1