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METHOD OF ISOLATION OF PURE CULTURE OF VASCULAR ENDOTHELIAL CELLS, MEDIUM FOR MAINTAINING CHARACTERISTICS OF VASCULAR ENDOTHELIAL CELLS, AND CULTURE METHOD INCLUDING SAME
METHOD OF ISOLATION OF PURE CULTURE OF VASCULAR ENDOTHELIAL CELLS, MEDIUM FOR MAINTAINING CHARACTERISTICS OF VASCULAR ENDOTHELIAL CELLS, AND CULTURE METHOD INCLUDING SAME
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机译:隔离血管内皮细胞纯培养方法,培养基维持血管内皮细胞特征,以及包括该血管内皮细胞特征的方法
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摘要
The present specification provides: a method of isolation of a pure culture of vascular endothelial cells, the method capable of isolating homogeneous endothelial cells adhered to a matrix for a specific time in a cell line of an endothelial cell lineage differentiated from human pluripotent stem cells; a medium for maintaining characteristics of vascular endothelial cells, comprising high-purity vascular endothelial cells isolated through the method, 4 ng/ml to 6 ng/ml of FGF2, 5 ng/ml to 10 ng/ml of EGF, 10 ng/ml to 30 ng/ml of VEGF-A, 20 ng/ml to 50 ng/ml of ascorbic acid, and DMEM/F-12 as active ingredients; and a culture method comprising same.
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