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MODIFIED GUIDE RNAS, CRISPR-RIBONUCLEOTPROTEIN COMPLEXES AND METHODS OF USE
MODIFIED GUIDE RNAS, CRISPR-RIBONUCLEOTPROTEIN COMPLEXES AND METHODS OF USE
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机译:改性指南RNA,CRISPR-核糖核酸蛋白复合物和使用方法
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摘要
Described herein are modified guide RNAs such as a single guide RNA including, from 5′ to 3′, a single-stranded protospacer sequence, a first complementary strand of a binding region for the Cas9 polypeptide, an aptamer that binds a biotin-binding molecule, and a second complementary strand of the binding region for the Cas9 polypeptide. Also described is an RNP complex including the modified guide RNA and a Cas9 polypeptide or active fragment thereof. Also included are methods of modifying target genes in cells using the modified guide RNAs.
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