A YEAST TWO-HYBRID RNA-PROTEIN INTERACTION SYSTEM BASED ON CATALYTICALLY INACTIVATED CRISPR-DCAS9

摘要

The inventors report here combining the use of CRISPR technology with the yeast two-hybrid protein-protein interaction system in order to create a highly advantageous, facile method for investigating RNA-protein interactions and roles of noncoding RNA in regulating gene transcription.