A system and method for sequencing a plurality of polynucleotidesA single DNA template moleculeThe step of attaching to each of the plurality of deposition elements on the sample containerStochastic optical switching chemical phenomenaSimultaneously applying all of the moleculesTo an attached moleculeOn events and off eventsEmitting up to four colors of fluorescence by stochastic optical switching andWhen an on event and an off event occur on an attached molecule,Imaging the on event and off events in real-time in color channels for each color andIncludingThe average distance between adjacent elements isShorter than Abbey's limit.Diagram
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