Method for genetically modified non human animal and complement dependent cell injury

摘要

It relates to genetically modified non-human animals and immunodeficient non-human animals characterized by the recovery of complement-dependent cytotoxicity, and methods and compositions for evaluating therapeutic antibodies in genetically modified immunodeficient non-human animals. In certain embodiments, the present invention is immunodeficient non-obese diabetes (NOD), A / J, A / He, AKR, DBA / 2, NZB / B1N, B10.D2 / oSn, and other mouse strains. The present invention relates to a gene that has been genetically modified to restore complement-dependent cytotoxicity that is lacking in unmodified immunodeficient mice. In another specific embodiment, the present invention ^{is, NOD.Cg-Prkdc scid IL2rg tmlWjl /SzJ(NSG),NOD.Cg-Rag1} tm1Mom Il2rg tmlWjl / SzJ (NRG), and ^{NOD.Cg-Prkdcscid} Il2rg tm1Sug / JicTac (NOG) relates to mice that have been genetically modified to restore complement-dependent cytotoxicity that is lacking in unmodified NSG, NRG, and NOG mice. Certain embodiments of the present invention provide methods for evaluating therapeutic or putative therapeutic antibodies in genetically modified immunodeficient mice characterized by an intact complement system.