FLUORESCENT POLYMER, FLUORESCENT PROBE AND CONJUGATION KIT FOR ADVANCED FUNCTIONAL ANALYSIS OF CELLS IN HAEMATOLOGY, IMMUNOLOGY AND MICROBIOLOGY, METHOD OF PREPARATION AND USE THEREOF

摘要

The present invention relates to a fluorescent polymer, comprising a linear semitelechelic statistical copolymer to which at least one fluorescent label is bound in an amount in the range of from 0.4 to 12 mol %, wherein the linear semitelechelic statistical polymer comprises polyacrylamide, polymethacrylamide, polyacrylate, polymethacrylate or poly (N- (2- hydroxypropyl)methacrylamide, wherein from 0.4 to 12 mol % of monomer units are replaced by monomer units of general formula (I) ( I), wherein R is selected from the group consisting of a straight or branched carbon alkylenyl chain with a number of carbons from 1 to 6, and -(CH₂)_q-(C(0)-NH-(CH₂)_r)_p-, wherein p=l to 5, and q and r are independently selected from 1, 2 and 3; Y is selected from the group consisting of a bond, - C(=0)-NH-, -NH-C(=0)-, -NH-C(=S)-NH-; the molecular weight Mn of the linear semitelechelic copolymer is in the range of from 10,000 to 100,000 g/mol; and the fluorophore has a molecular weight in the range of from 350 to 1,500 g/mol, an excitation wavelength in the range of from 300 to 850 nm and an emission wavelength in the range of from 350 to 1,200 nm. The present invention further relates to a fluorescent probe, a conjugation kit, a method of their preparation and to the use thereof.