Effective NK cell culture methods with NK Cell Culture Media Kit

摘要

The present invention relates to a method of culturing natural killer cells (NK cells) applied to immunotherapy, and more specifically, NK cells excellent in the treatment of malignancies by culturing lymphocytes derived from human peripheral blood. It is about the NK cell culture method that efficiently amplifies and activates and the culture kit used (NKCM Kit). The method of amplifying NK cells of the present invention includes a step of stimulating NK cells by separating lymphocytes from peripheral blood, and IL-2, IL-12, IL-15, IL-17, IL-18, and IL-21. It consists of a step of culturing in a medium and a step of separating NK cells. The present invention provides a pharmaceutical composition for cell therapy comprising NK cells produced by the method of amplifying NK cells of the present invention. The pharmaceutical composition for cell therapy of the present invention is sometimes used to treat infectious diseases and/or cancer.