METHOD FOR USING WHOLE GENOME RE-SEQUENCING DATA TO QUICKLY IDENTIFY TRANSGENIC OR GENE EDITING MATERIAL AND INSERTION SITES THEREOF

摘要

Provided is a method for using whole genome re-sequencing data to quickly identify transgenic or gene editing material and insertion sites thereof, said method comprising: extracting genomic DNA; obtaining paired-end sequencing data of a whole genome; determining whether the sequence of an expression vector containing a T-DNA sequence, inserted in a plant to be sequenced, is known; determining whether there is a transgenic event or a gene editing event in the plant to be sequenced, and whether a backbone sequence transfer event has occurred; determining the insertion site of the T-DNA sequence; the method combines bioinformatic analysis means, and if an expression vector is known or unknown, it is determined whether a transgenic or gene editing event has occurred; if the expression vector is known, then not only can the precise location, direction, copy number, and flanking sequence information of the target sequence inserted into the genome be quickly and accurately given, but it is also determined whether there is a backbone sequence which is a sequence other than the target sequence inserted into the genome, and the same positioning is also provided.