CHIMERIC ANTIGEN RECEPTOR T CELL TARGETING EGFRVIII AND INTERFERING WITH IL-6 EXPRESSION, PREPARATION METHOD THEREFOR AND APPLICATION THEREOF

摘要

Disclosed is a chimeric antigen receptor T cell targeting EGFRvIII and interfering with IL-6 expression, comprising a chimeric antigen receptor CAR-EGFRvIII targeting EGFRvIII, and siRNA interfering with IL-6 expression. The CAR-EGFRvIII comprises amino acid sequences of a single-chain antibody targeting EGFRvIII, an extracellular hinge region, a transmembrane region, an intracellular signal region connected in sequence from an amino terminal to a carboxyl terminal. The single-chain antibody targeting EGFRvIII comprises an amino acid sequence as represented by SEQ ID NO: 1. A DNA sequence corresponding to the siRNA interfering with IL-6 expression comprises at least one of nucleotide sequences as represented by SEQ ID NO: 2, SEQ ID NO: 3, and SEQ ID NO: 4.