Method of storage, in the fresh state, the seeds and fruits to water content of less than 60 approximately, and in particular for kola nut and chestnuts

• 1. 一种用于储存60％含水量的污泥料仓 [P] . 中国专利： CN213084200U . 2021-04-30
• 2. 一种在含水量大于60％的软土上进行基坑开挖的方法 [P] . 中国专利： CN108343067B . 2020.02.07
• 3. METHODS OF OBTAINING A COMPLETE STABILIZED EXTRACT OF FRESH KOLA NUTS HAVING A HIGH COMBINED CAFFEINE CONTENT [P] . 英国专利： GB1316036A . 1973-05-09

  机译： 获得具有高结合咖啡因含量的新鲜可乐果的完整稳定提取物的方法

• 4. Method of storage in the fresh state of the kola nut [P] . 法国专利： FR1113426A . 1956-03-29

  机译： 在可乐果新鲜状态下储存的方法

• 5. methods for preserving fresh fruits, vegetables, nuts, seeds and eggs. [P] . NL161974C . 1980-04-15

  机译： 保存新鲜水果，蔬菜，坚果，种子和鸡蛋的方法。

• 1. Changes of Endogenous Hormone Contents in Fruit, Seeds and Their Effects on the Fruit Development of Rosa roxburghii [J] . FAN Wei-guo, AN Hua-ming, LIU Guo-qin, 农业科学学报（英文版） . 2003,第012期
• 2. Effect of Several Common Processing Methods on Content Changes of Resistant Starch and Main Nutrients in ‘Luotian Chestnut＇ [J] . Linling LI, Bingjie HUANG, Hua CHENG, 农业生物技术：英文版 . 2018,第005期
• 3. Consumers' Liking toward Roasted Chestnuts from Fresh and Frozen Nuts.Influence of Psycho-Social Factors and Familiarity with Product [C] . International chestnut symposium . 2009
• 4. Fresh Fruits and Vegetables in a Rural Elementary School: A Mixed-Methods Program Evaluation. [D] . Moore, Patricia M. 2016
• 5. The vitamin E derivative garcinoic acid from Garcinia kola nut seeds attenuates the inflammatory response [O] . Maria Wallert, Julia Bauer, Stefan Kluge, 2019
• 6. The Effect of Short-term Storage and the Hot Water Treatment of Fresh-Cut Pepper Fruit cv. ‛Blondy F1’ and ‛Yecla F1’ on the Content of Bioactive Compounds and Antioxidant Properties [O] . Szwejda-Grzybowska Justyna I., Kosson Ryszard, Grzegorzewska Maria 2016
• 7. Analysis of Specialized Pesticide Problems Invertebrate Control Agents - Efficacy Test Methods Volume Ⅰ. Foliar Treatments Ⅰ(Deciduous Fruit Trees, Small Fruits, Citrus & Subtropical Fruits, Tree Nuts) [R] . Glenn Carman, Angus Howitt, Robert Horsburgh 1977

• 1. Storage of Campomanesia adamantium(Cambess.)O.Berg Seeds:Influence of Water Content and Environmental Temperature [J] . Daiane Mugnol Dresch ,Silvana de Paula Quintao Scalon ,Tathiana Elisa Masetto . 美国植物学期刊（英文） . 2014,第17期
• 2. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 3. Comparison of Sugar Content in Bottled 100 Fruit Juice versus Extracted Juice of Fresh Fruit [J] . Jasmine Y. Serpen . 食品与营养科学（英文） . 2012,第11期
• 4. 贮藏温度和种子含水量对毛竹种子生活力保存的影响 [C] . 蔡春菊 ,刘凤 ,彭镇华 . 第三届全国生物质材料科学与技术学术研讨会 . 2009
• 5. 成熟期土壤含水量对‘北红’和‘北玫’葡萄果实酚类物质积累的影响 [A] . 李倩 . 2019