Radio immuno logical analytical methods for the determination of the total - thyroxine in a serum sample

摘要

An immunoassay for the measurement of total thyroxine in blood serum which employs trichloracetic acid and base to precipitate or denature the protein to quantitatively extract thyroxine. This procedure along with the use of radioactively labeled T.sub.4 antiserum, and the resin sponge to separate free and bound hormone, minimizes the number of steps required in the assay, which can be performed in the same tube in less than ninety minutes.