Antigenic extracts from Pseudomonas aeruginosa - with bacterial metabolites removed before extraction, used for making vaccines and antisera

摘要

Process for recovering the active principle (A) of the vaccine or serum against Pseudomonas aeniginosa comprises (i) culturing, pref. in the absence of impurities in the culture medium, a vinilent strain of the bacterium of morphological type 1a; (ii) collecting the cells, removing any culture medium impurities if necessary; (iii) washing with a liq. of low ionic strength, such as distilled water, to remove the labile bacterial excretion products; (iv) suspending the washed cells in a liq. and heating the suspension to 37-80 degrees C at which temp. the medium has sufficient ionic strength to extract the components (peribaccilary substances) from the capsule surrounding the bacterium, then (v) removing solids and collecting the soln. of (A). Vaccines contg. (A) and antisera obtained from animals inoculated with (A) are also new. (A) are at least as active as previously prepared antigen extracts but since the bacterial excretion products, esp. extracellular enzymes, have been removed they are much less toxic. The vaccines/antisera can be used for prophylaxis/treatment of humans and animals.