VECTORS, DERIVED FROM LAMBDA BACTERIOPHAGUS, ALLOWING THE INSERTION AND EXPRESSION OF A GENE, PROCARYOTE OR EUKARYOTE, UNDER THE CONTROL OF THE PROMOTER AND THE OPERATOR OF THE LACTOSE OPERON OF ESCHERICHIA COLI BACTERIA

摘要

P Modified lambda bacteriophage DNA. It comprises mutations Qam73 and Sam7 having the effect of preventing or delaying the expression of late genes, a DNA fragment comprising at least part of the Z gene of Escherichia coli and a promoter of a bacterial operon, in particular of the lactose operon in a non-essential part of the genome of the phase, in the Z gene, a cleavage site by an endonuclease such as Ecori, to the exclusion of any other site of the same type in phage DNA. /P P Application to induction by a bacteria of directed protein synthesis. /P