Thrombolytic compsns. of purified urokinase - esp. contg. urokinase I made by chromatography on CMC

摘要

Urokinase with mol.wt. of 33000 (urokinase I) substantially free of urokinase of mol. wt. about 55000 (urokinase II) having an activity in the fibrin clot test of 50000 uCTA/mg of proteins and activities on AGLME substrate and fibrin plague of 150000 uCTA/mg of protein, its purity being such that dissolution in 0.04 M (NH4)2SO4 soln. at pH 6 and contacting it with a Sepharose (RTM) carboxymethyl gel chromatography column and linear gradient elution to obtain an eluate at pH 6 with an ammonium sulphate content of 0.25 M permits isolation of urokinase with substantially a single peak. Medicinal compsn. for thrombolytic treatment as the first component, a modified plasminogen, and as the second urokinase I having a single peak of activity. Urokinase I or urokinase II, each substantially free of the other, may be injected or perfused for treatment of blood disorders, e.g. deep venous thrombosis. Lower doses may be given of a specific type and the dose may be better tolerated by the patient.