Spore-less Basidiomycetes strains prepn. - by pairing a spore-less strain with a wild strain, and pairing the next two generations

摘要

Sporeless strains of Basidiomycetes can be prepd. by (a) pairing a strain with a mutation for sporelessness (sp-) with a wild strain; (b) allowing the di- or hetero-karyon obtd. to fruit and collecting the spores; (c) allowing the obtd. spores to germinate individually; (d) pairing the homokaryons obtd. in (c) with test homokaryons for sporelessness and bringing the di- or heterokaryons produced to fruiting; (e) examining the fruiting bodies for sporelessness and determining which homokaryons used in (d) for pairing contained the sp- mutation; and (f) pairing to homokaryons contg. sp- to give new di- or heterokaryons, which are then brought to fruiting and the fruiting bodies finally tested for sporelessness. The most important edible fungi are Basidiomycetes, but the wild strains produce large amounts of sexual spores which can serve as vehicles for viruses, can cause allergies in humans and animals and, in the case of the species which destroy wood, can damage the environment.