MEASURING METHOD OF ELECTRIC POTENTIAL OF ERYTHROCYTE MEMBRANE

摘要

PURPOSE:To measure electric potential of a membrane of an erythrocyte safely, surely, quickly and accurately, by measuring the electric potential of the membrane of the erythrocyte after one kind or more of hemoglobin, an erythrocyte cell component and blood plasma component are added in advance to a fat soluble ion solution and adding the correction of a bias etc. by the volume of water in the erythrocyte to this electric potential. CONSTITUTION:Before adding an erythrocyte or erythrocyte suspension to a mixed solution of a fat soluble ion such as tetraphenyl phosphonium (TPP+) etc. and NaCl-Tris buffer solution, cattle hemoglobin available on the market, an erythrocyte cell component or preferably one kind or more of blood plasma of an animal itself or the same kind animal gathered blood in order to obtain the erythrocyte, are added to the solution of adid TPP+ at a fixed rate. Next, the sample erythrocyte or erythrocyte suspension is added and electric potential of a membrane is measured by using a fat soluble ion selective electrode. Then, the correction of a bias basing on the volume of water in the erythrocyte and that basing on the adsorption quantity of the TPP+ for the erythrocyte, are added to this electric potential of the membrance. In this manner, the electric potential of the membrane having no variance is measured without using a dangerous radioactive element and diagnosis and early stage discovery of a primary cause of apoplexy are made.