REDUCTION OF IRON (3) PORPHYRIN COMPLEX

摘要

PURPOSE:An enzyme system consisting of NADP, D-glucose-6-phosphoric acid, glucose-6-phosphoric acid dehydrogenase, ferredoxin, and ferredoxin-NADP reductase is made to act to reduce the iron to divalent state in the titled complex. CONSTITUTION:In an aqueous medium such as water or a phosphate buffer solution, a reducing agent is made to act on an iron (III) porphyrin complex such as protoporphyrin IX, duteroprophyrin IX, mesoporphyrin IX or hematoporphyrin IX. This reducing agent is composed of an enzyme system containing NADP, D-glucose-6-phosphoric acid, glucose-6-phosphoric acid dehydrogenase, ferredoxin and ferredoxin-NADP reductase and may contain catalase additionally. The amount of D-glucose-6-phosphoric acid is more than 1mol per mol of the iron (III) porphyrin complex and the aqueous medium is adjusted to 5-9 in pH.