MICROBIOLOGICALLY PREPARED POLYPEPTIDE WITH THE AMINO ACID SEQUENCE OF PROINSULIN OF A PRIMATE DNA AND PLASMIDS WHICH CODE FOR THIS SEQUENCE MICROORGANISMS WHICH CONTAIN THIS GENETIC INFORMATION AND PROCESSES FOR THEIR PREPARATION

摘要

The process for the preparation of the polypeptide which contains the amino-acid sequence of the proinsulin of primates entails the genetic information for the biosynthesis of the said polypeptide being obtained by means of mRNA from primates, the cDNA being prepared therefrom and inserted into a plasmid, the resulting hybrid plasmid being used to transform microorganisms, and the microorganisms producing the polypeptide being obtained by selection and cultivation.