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Factor VIII concentrates prepared from heparinized plasma by the application of a cold precipitation technique
Factor VIII concentrates prepared from heparinized plasma by the application of a cold precipitation technique
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机译:通过冷沉淀技术从肝素化血浆中制备因子VIII浓缩物
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摘要
A method for recovering Antihemophilic Factor VIII from whole blood or blood plasma or blood plasma fractions freshly collected directly into an anticoagulant selected from the group of heparin, sodium heparin, or mixtures thereof, the heparin being used in the range of 6-8 units/ml of plasma or 3.5-4 units/ml of blood; and either first separating the red cells from the plasma and/or freezing the heparinized plasma, resolubilizing the plasma, isolating a cryoprecipitate from the plasma, resolubilizing the cryoprecipitate, adding a saline heparin solution to the resolubilized cryoprecipitate, incubating the resolubilized cryoprecipitate at a temperature of from about 0° C. to about 4. degree. C. for a time of at least 2 hours, whereby Factor VIII present in the cryoprecipitate is insolubilized using heparin precipitable cold insoluble globulin and the resulting Factor VIII rich precipitate also includes cold insoluble globulin, separating the Factor VIII rich precipitate, and isolating Factor VIII therefrom. This new process permits recovery of a high purity Factor VIII preparation which can easily be made in the blood bank or alternatively, the cold precipitation step can be carried out in a fractionation plant.
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