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Process for the preparation of human tissue plasminogen activator DNA sequence coding for this activator, replicable expression vector for the DNA sequence and the microorganism or cell culture transformed with the expression vector
Process for the preparation of human tissue plasminogen activator DNA sequence coding for this activator, replicable expression vector for the DNA sequence and the microorganism or cell culture transformed with the expression vector
Human tissue plasminogen activator (t-PA) is produced in useful quantities using recombinant techniques. This invention thus enables production of t-PA free of contaminants associated with it in its native cellular environment. Methods, expression vehicles and various host cells useful in its production are also disclosed.
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