A procedure for obtaining Mutants of Clostridium acetobutylicum.

摘要

Procedure for obtaining Mutants of Clostridium acetobutylicum.Consists of the following Operations performed under Anaerobic Conditions: a) extending aqueous Culture of a Strain of Clostridium acetobutylicum on the surface of a Solid Culture Medium containing n-butanol; (b) provide a mutagenic Agent on the surface of the growing medium; (c) REC Oger is a part of the Colony of Mutants formed when the concentration of n-butanol is equal toStarting and Development of the bacterial Strain is observed. The agents used are: metilsulfonato mutogenos Ethyl, N - methyl - N - nitro - N - nitrosogranidi na, the ICR 191, Nitrous acid and N - Oxide nitroquinolina.tiene application to obtain n-butanol.