METHOD FOR MEASURING CONCENTRATION OF ADENOSINE 5'-DIPHOSPHATE AND ENZYME ACTIVITY OF ENZYME FORMING ADENOSINE 5'-DIPHOSPHATE

摘要

PURPOSE:To measure the concn. of adenosine 5'-diphosphate (ADP) and the enzyme activity of enzyme forming ADP by using an enzyme electrode having two kinds of immobilized enzyme; immobilized pyruvate oxidase and immobilized pyruvate kinase. CONSTITUTION:An immobilized pyruvate oxidase film 2 and an immobilized pyruvate kinase film 3 are fixed on the surface of an oxygen electrode or hydrogen peroxide electrode 1 to constitute an enzyme electrode. A prescribed amt. of sample is added from a supply pipe 9 to a buffer soln. 3 in the case of measuring the concn. of ADP in the sample. ADP and phosphenol pyruvic acid are diffused in the film 3 to form a pyruvic acid. The pyruvic acid is diffused and oxidized in the film 2 and in this stage said acid consumes oxygen and generates hydrogen peroxide. The hydrogen peroxide is detected by the change in the current of the electrode 1. The intended enzyme substrate is added preliminarily to the buffer soln. and the similar measurement is performed in the case of measuring the enzyme activity of the enzyme forming the ADP in the sample.