Culture medium without serum of animal cells, without hormones and growth factors and by a primary culture and of the production of cell lines using this medium.

摘要

The invention relates to a culture medium of animal cells, without serum, essentially comprises: a basic medium a.d' synthetic designed for the culture of animal cells; b.de 2 to 30 meq1 of fatty acids or their esters, in the proportions reported at the free acid: 0 to 24 mmole1 of palmitic acid; 0,6 has 9mmole1 of cis - palmitoleic acid; 0 to 2mmole1 of stearic acid; 0,5 has 10mmole1 of cis - oleic; 1,2 has 27mmole1 of cis - linoleic acid; and 0,3 has 4mmole1 of cis - linolenic acid, adsorbed on c.au least one lipophilic biopolymer in a quantity which is capable of ensuring the dissolution of the fatty acids or esters thereof presents in the medium which is essentially devoid hormones and growth factors. / p & & p & this medium is particularly well adapted both to the primary culture, as in the production of epithelial cell lines of the liver.