METHOD FOR THE CULTIVATION OF NORMAL DIPLOID CELLS AND CULTIVATION MEDIUM USED THEREFOR

摘要

A method for the multiplication of normal diploid cells from animals is described, comprising cultivating the cells in a culture medium containing an amine derivative or derivatives represented by the general formula: NR1R2R3 (wherein R1, R2 and R3 represents a straigh, branched or cyclic alkyl group or a hydrogen atom, provided that R1, R2 and R3 are not hydrogen atoms at the same time. Addition of these specific amine derivatives permits multiplication of normal diploid cells in a culture medium containing no serum or only a limited concentration of serum. These amine derivatives are inexpensive and can be stored for a long period of time, and their storage and handling are easy. The use of these amine derivatives overcomes various problems encountered in using serum in the multiplication of normal diploid cells according to conventional methods.